FIG.3.

Profiles of light emission were monitored during prolonged growth of Y. enterocolitica luxCDABE transposon mutants at 10°C (solid diamonds) and 30°C (open diamonds). Selected diagrams show significant decreases (A) or increases (B to J) in promoter activity in response to temperature decline. Absolute RLU values, instead of corrected light emission values considering the temperature effect, were used for the graphs. (J) The dashed line in the diagram of the cspA::luxCDABE mutant shows the relative amount of major cold shock proteins (MCSPs) following a cold shock from 30°C to 10°C, as determined by two-dimensional gel electrophoresis, with the maximum level of relative MCSP units per cell set at 100% (47).