TABLE 2.
Primers used in this study
| Target gene | 5′-3′ sequencee | Modification | Template |
|---|---|---|---|
| Sequencing by inverse PCR | |||
| GTGCAATCCATTAATTTTGGTG | SspIc | ||
| GAGCATGCGCCATCTTATGC | ClaIc | ||
| GCGCATCGGGCTTCCCATAC | ClaIc | ||
| GGCGGCCTATTCTTTAGTTC | HindIIIc | ||
| TGTTCCGTTGCGCTGCCCGGa | ClaIc | ||
| GTTGCGCTGCCCGGATTACAGa | HindIIIc | ||
| GAAATCACCATGAGTGACGACTG | HindIIIc | ||
| CATACGTATCCTCCAAGCCb | DraI,c SspIc | ||
| GTACCGAGCTCGAATTCGCGb | ClaIc | ||
| GTCATACGTATCCTCCAAGCb | HindIII,c SphIc | ||
| CAGATGCGATTAATTGGGCG | SphIc | ||
| GAGCAAATATTGCCTTATG | DraIc | ||
| Construction of pKRG knockout vectors | |||
| uhpC | CGATGAGCTCCCGATAATGCCA | SacId | |
| uhpC | GCGATCTAGACGATAACGTGCC | XbaId | |
| Test primer | CCACTTACCCACACTTGG | ||
| YE2575 | CGATGAGCTCATCATAGCATCAGCG | SacId | |
| YE2575 | GCGATCTAGATCACCAGCTTGC | XbaId | |
| Test primer | GCAGCAATTTCTGATGCG | ||
| ybcM | CGATGAGCTCTCAAGCATACGG | SacId | |
| ybcM | GCGATCTAGAGTCATTCGCTGG | XbaId | |
| Test primer | GGTCAATTGACAGGCAGC | ||
| Test primer | CCTGTTCAGCTACTGACG | pKRG9 | |
| Test primer | GTACTAAGCTCTCATGGC | pKRG9 | |
| Construction of pACYC184 complementation vectors | |||
| Test primer | GGTATTCACTCCAGAGCG | pACYC184 | |
| Test primer | ACCAGACCGTTCAGCTGG | pACYC184 | |
| YE2575 | CCTAGTACTCCGTATCGCTAACACC | ScaId | |
| YE2575 | CCTAGTACTGGATACCCAACTGAGG | ScaId | |
| uhpC | CCTGAATTCTCAAGCTTCAGCTTGTTTC | EcoRId | |
| uhpC | CCTGAATTCGGTGTATTGCACTGGC | EcoRId | |
| Cloning of gfp promoter probe vectors | |||
| cspB | GCATGTCGACCCATGTCTGATG | SalId | |
| cspB | CCGGAATTCGCCAATAGATGG | EcoRId | |
| gltP | GCATGTCGACCGCTACGTGATT | SalId | |
| gltP | CCGGAATTCCTTTGCATCCCA | EcoRId | |
| YE2463 | GCATGTCGACGGTTATAAGACGAATACC | SalId | |
| YE2463 | CCGGAATTCTTAATAAAATTAACCCGCG | EcoRId | |
| YE2537 | GCATGTCGACCCCAGAGTGCTT | SalId | |
| YE2537 | CCGGAATTCGCCAAGCTGAAT | EcoRId | |
| YE2575 | TAATGTCGACTAATGGGGTCAG | SalId | |
| YE2575 | GTTAGAATTCCATAAGTACGTC | EcoRId | |
| YE2848 | GCATGTCGACGCATGCACTTACCCG | SalId | |
| YE2848 | CCGGAATTCGGTGAAAGATTGTGCTG | EcoRId | |
| YE2922 | GAAAGTCGACTAATCTCCTCTTG | SalId | |
| YE2922 | TTAAGAATTCGAATGTTTTTCTTTGC | EcoRId | |
| YE0480 | CAACGTCGACAGGTGGTTCTAATGGC | SalId | |
| YE0480 | GCGTGAATTCGCGCACATTAAATTATAATTTTA | EcoRId | |
| YE0951 | GCATGTCGACGCGATGATAAAAACAGGG | SalId | |
| YE0951 | CCGGAATTCCACAAGAGCACTCACC | EcoRId | |
| YE0960 | GCATGTCGACGACTTCTGAGTGCTGG | SalId | |
| YE0960 | CCGGAATTCTGTGGCTGCACATGG | EcoRId | |
| Test primer | TCCGTATGTTGCATCACC | pPROBE-NT | |
| Test primer | GATCGGAAGCTTGCATGC | pPROBE-NT |
a
I-end sequencing.
b
O-end sequencing.
c
pUTmini-Tn5 luxCDABE Km2.
d
Chromosomal DNA of Y. enterocolitica W22703.
e
Recognition sites of restriction enzymes are underlined.