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. 2006 Apr;188(8):2945–2958. doi: 10.1128/JB.188.8.2945-2958.2006

TABLE 3.

Long-term response of Y. enterocolitica W22703 to low temperaturesa

Insertion site Gene Homologue Protein specification Gene fusion structure Induction (OD405) Classification Response ratio
Up-regulated genes
    3387325 YE3114b ymoAb Modulator YmoA P-luxCDABE-ymoA 0.19 Regulation 123
    4168711 YE3823c cspAc Cold shock protein A P-cspA′-luxCDABE 0.20 Regulation 84
    1066450 YE0940 cspB Cold shock protein B P-cspB′-luxCDABE 0.28 Regulation 268
    368636 YE0310 gltP Glutamate-aspartate symport protein P-gltP′-luxCDABE 0.29 Transport 46
    3033404 YE2793 Unknown protein P-YE2793′-luxCDABE 0.33 33
    683797 YE0595 arcA Aerobic respiration control protein ArcA P-arcA′-luxCDABE 0.37 Regulation 8
    4470965 YE4087 uhpC Hexose phosphate transport system regulatory protein P-YE4090-uhpA-uhpB-uhpC′-luxCDABE 0.41 Regulation 36
    2732143 YE2537 Flagellar basal body M-ring protein P-YE2537′-luxCDABE-fliG-fliH-fliI 0.50 Motility 33
    1612069 YE1436 Putative transcription regulatory protein P-YE1436′-luxCDABE 0.52 Regulation 33
    Strain specific No homology 0.53 6
    2898299 YE2673 Putative chemotaxis signal transduction protein P-YE2673-luxCDABE 0.53 Motility/signaling 801
    2722746 YE2527 ybcM Putative AraC type regulatory protein P-ybcM′-luxCDABE 0.54 Motility 176
    2649603 YE2463 Putative outer membrane porin P-YE2463′-luxCDABE 0.54 Transport 41
    2248595 YE2057 ssrAB-activated, putative virulence factor SrfA P-YE2057′-luxCDABE-YE2058-YE2059 0.54 Virulence 25
    2259046 YE2063 Hypothetical membrane protein P-YE2063′-luxCDABE 0.55 73
    2772356 YE2577 cheA Chemotaxis protein P-motA-motB-cheA′-luxCDABE-cheW 0.57 Regulation 335
    Strain specific No homology 0.58 256
    2716443 YE2521 fleC Flagellin FleC P-fleA-fleB-fleC′-luxCDABE 0.59 Motility 27
    2769982 YE2575 Methyl-accepting chemotaxis protein P-YE2575 (MCPI)′-luxCDABE-tap(MCPIV)-cheR-cheB 0.60 Motility/signaling 32
    2722197 YE2526 fliT Repressor of class II promoters P-fliT′-luxCDABE 0.60 Motility 22
    Strain specific tcaB Putative toxin subunit P-tcaB′-luxCDABE 0.65 Virulence 81
    2721668 YE2525 fliS Putative cytoplasmic chaperone P-luxCDABE-fliS 0.65 Motility 8
    4432406 YE4063 Hypothetical protein with EAL domain P-YE4063′-luxCDABE 0.66 Signaling 1,147
    Strain specific Hypothetical protein 0.67 26
    1476057 YE1324 Hypothetical protein with EAL domain (signal transduction) P-YE1324′-luxCDABE 0.73 Signaling 98
    1074733 YE0951b Urea amidohydrolase P-luxCDABE-YE0951-yeuB-ureC-ureE-ureF 0.74 Metabolism 17
    2783524 YE2586 Mg2+ transport ATPase protein C mgtC P-luxCDABE-YE2586 0.75 Metabolism 8
    2715679 YE2520 fliB Lysin-N-methylase P-fliB′-luxCDABE 0.75 Motility 150
    Strain specific tcaA Putative toxin subunit P-tcaA′-luxCDABE 0.77 Virulence 12
    1086842 YE0964 Probable membrane protein P-YE0964′-luxCDABE-YE0963 0.79 6
    1084151 YE0960d Urea operon P-YE0959-YE0960′-luxCDABE 0.81 Metabolism 12
    3177216 YE2922 Alkaline serine protease (subtilisin) P-YE2922′-luxCDABE 0.83 Metabolism 38
    4369922 YE4013 1,4-α-glucan branching enzyme GlgB P-luxCDABE-YE4013-YE4012-YE4011 0.83 Metabolism 26
    3095861 YE2848 Putative chemotaxis methyl-accepting transducer P-YE2848′-luxCDABE 0.84 Signaling 95
    555157 YE0480 Transport of large molecules/secretion protein; Fhac homologue P-YE0480′-luxCDABE 0.84 Virulence 58
    3292901 YE3021 Histidine ammonia-lyase (histidase), HutH P-YE3021′-luxCDABE-YE3020 0.89 Metabolism 13
    4037041 YE3697 Permease of the drug/metabolite transporter (DMT) superfamily P-YE3697′-luxCDABE 0.90 Metabolism 15
Down-regulated genes
    4116172 YE3774 Protein-Npi-phosphohistidine-sugar phosphotransferase P-YE3774′-luxCDABE-YE3773 0.98 Metabolism 0.6
    4509439 YE4121 Sugar-binding periplasmic protein P-YE4121′-luxCDABE-YE4120 (sugar transport ATP-binding protein)-YE4119 (d-xylose ABC transporter)-YE4118 (probable AraC family transcription regulatory protein) 1.00 Metabolism 0.5
    4562537 YE4164 Heat shock protein P-YE4164′-luxCDABE 0.84 0.2
    p5086 Not annotated ND 0.2
    Strain specific Prophage P2 OGR protein 0.87 0.3
a

The first column depicts the transposon insertion with respect to the genome sequence of Y. enterocolitica 8081. Mutagenized genes are indicated by their number taken from the Y. enterocolitica annotation at the Sanger Institute homepage (column 2). Gene names and further protein specifications were obtained from BLAST analysis (columns 3 and 4). The prime symbols in column 5 mark truncated genes. ND, not determinable.

b

Mini-Tn5 luxCDABE genes were inserted in opposite to the putative promoters of ymoA and YE0951.

c

Transposon insertion was in strain NCTC10460.

d

As shown by a gfp fusion construct, the luxCDABE insertion is transcribed from the promoter upstream of ureD.