TABLE 3.
Genes up- and down-regulated in testes from Tslc1−/− mice
| Gene product | Accession no. | Microarray analysis results
|
Quantitative RT-PCR analysis resultsb
|
P valuec | |||
|---|---|---|---|---|---|---|---|
| Signal intensitya
|
Ratio of Tslc1−/− value/Tslc1+/+ value | Tslc1−/− value | Tslc1+/+ value | ||||
| Tslc1−/− | Tslc1+/+ | ||||||
| Up-regulated genes in Tslc1−/− testes | |||||||
| Phospholipase A2, group XIIA (Pla2g12a) | AI845798 | 13,102 | 771 | 17.0 | 11 ± 2.1 | 4.1 ± 0.8 | 0.03 |
| Purine-nucleoside phosphorylase (Pnp) | U35374 | 1,629 | 410 | 4.0 | 6.1 ± 0.7 | 2.5 ± 0.6 | 0.02 |
| Mus musculus cDNA 5′ end clone | AA874329 | 706 | 272 | 2.6 | |||
| FUS interacting protein 1 (Fusip1) | AF060490 | 695 | 282 | 2.5 | |||
| Hemoglobin, beta adult major chain (Hbb-b1) | J00413 | 1,446 | 636 | 2.3 | |||
| DnaJ homolog, subfamily A, member 2 (Dnaja2) | AA763945 | 1,833 | 860 | 2.1 | 470 ± 160 | 300 ± 120 | NS |
| Vascular cell adhesion molecule 1 (Vcam1) | U12884 | 2,321 | 1,546 | 1.5 | 110 ± 5.8 | 38 ± 2.5 | 0.0002 |
| Guanylate kinase 1 (Guk1) | U53514 | 3,225 | 2,294 | 1.4 | 23 ± 12 | 4.4 ± 0.1 | NS |
| Angiopoietin-like 4 (Angpt14) | AF110520 | 6,143 | 4,823 | 1.3 | 3.1 ± 0.2 | 1.2 ± 0.2 | 0.002 |
| Down-regulated genes in Tslc1−/− testes | |||||||
| Immunoglobulin superfamily 4 (Igsf4/Tslc1) | AF061260 | 444 | 3,943 | 0.11 | 0 ± 0 | 100 ± 18 | 0.001 |
| Immunoglobulin superfamily 4 (Igsf4/Tslc1) | AB021966 | 690 | 3,739 | 0.18 | |||
| Platelet/endothelial cell adhesion molecule 1 (Pecam1) | L06039 | 181 | 799 | 0.23 | 1.6 ± 0.2 | 1.2 ± 0.3 | NS |
| Mus musculus cDNA 3′ end clone | AW047207 | 241 | 895 | 0.27 | |||
| Peroxiredoxin 2 (Prdx2) | U20611 | 281 | 892 | 0.31 | 16 ± 3.0 | 11 ± 0.6 | NS |
| Dehydrogenase/reductase X chromosome (Dhrsx) | AI846822 | 4,509 | 11,760 | 0.38 | 11 ± 1.6 | 12 ± 2.1 | NS |
| Ornithine decarboxylase antizyme 3 (Oaz3) | AB016275 | 17,735 | 44,962 | 0.39 | 94 ± 6.8 | 150 ± 9.2 | 0.007 |
| Splicing factor, arginine/serine-rich 16 (Sfrs16) | AF042799 | 430 | 1,030 | 0.42 | |||
| Protein phosphatase 2, regulatory subunit B, beta isoform (Ppp2r2b) | AW048155 | 6,134 | 14,358 | 0.43 | 9.2 ± 1.9 | 23 ± 1.5 | 0.004 |
| Mouse endogenous murine leukemia virus modified polytopic provirus DNA | M17327 | 789 | 1,769 | 0.45 | |||
| Deleted in polyposis 1-like 1 (Dplll) | AA755260 | 14,224 | 29,836 | 0.48 | 340 ± 30 | 970 ± 130 | 0.008 |
| Lysophospholipase 1 (Lypla1) | U89352 | 6,971 | 14,600 | 0.48 | 7.8 ± 0.4 | 5.6 ± 1.6 | NS |
| Suppressor of K+ transport defect 3 (Skd3) | U09874 | 7,709 | 15,612 | 0.49 | |||
| S100 calcium binding protein A13 (S100a13) | X99921 | 773 | 1,430 | 0.54 | |||
| Protamine 1 (Prm1) | Z47352 | 18,600 | 31,700 | 0.58 | 12,000 ± 2,200 | 16,000 ± 1,700 | NS |
| RAN GTPase activating protein 1 (Rangap1) | U20857 | 4,960 | 8,480 | 0.58 | 72 ± 33 | 110 ± 47 | NS |
| Growth arrest-specific protein 6 (Gas6) | X59846 | 1,120 | 1,820 | 0.61 | 80 ± 8.2 | 170 ± 35 | 0.04 |
| Bcl2-associated athanogene 1 (Bag1) | AF022223 | 3,412 | 5,546 | 0.61 | 0.1 ± 0.1 | 1.1 ± 0.4 | NS |
| Cyclin-dependent kinase inhibitor 1C (Cdkn1c) | U22399 | 1,090 | 1,650 | 0.66 | 2.3 ± 0.2 | 1.8 ± 0.1 | NS |
| Mothers against decapentaplegic homolog 6 (Smad6) | AF010133 | 2,070 | 2,930 | 0.70 | 79 ± 11 | 94 ± 8.4 | NS |
| Sperm mitochondrion-associated cysteine-rich protein (Smcp) | M88463 | 29,700 | 38,900 | 0.76 | 430 ± 43 | 930 ± 81 | 0.002 |
a
Average value of two independent experiments.
b
The Amount of Igsf4/Tslc1 in the Tslc1+/+ testis was assigned a value of 100. Data are average values ± SE of three to five independent experiments.
c
NS, not significant.