FIG. 3.

Ubp8p and Sgf11p are mutually dependent on each other for recruitment to the GAL1 UAS. (A) Recruitment of Ubp8p to the GAL1 UAS requires Sgf11p. Wild-type and SGF11 deletion mutant strains expressing multiple-Myc-epitope-tagged Ubp8p were first grown in glucose-containing (YPD) medium and then shifted to galactose-containing (YPG) medium 4 h before treatment with formaldehyde. Immunoprecipitations were performed as described in the legend to Fig. 1. A primer pair targeting the GAL1 UAS was used for PCR analysis of the immunoprecipitated DNA samples. (B) Recruitment of Sgf11p to the GAL1 UAS is dependent on Ubp8p. Wild-type and UBP8 deletion mutant strains expressing multiple-Myc-epitope-tagged Sgf11p were grown as described above before treatment with formaldehyde. Immunoprecipitation and PCR analysis were performed as described above. (C) Sgf11p is dispensable for recruitment of SAGA to the GAL1 UAS. Wild-type and SGF11 deletion mutant strains were grown, cross-linked, and immunoprecipitated as described above.