FIG. 7.

Ubp8p does not regulate H3-K4 methylation at the ADH1 and CUP1 genes. (A) Analysis of di- and trimethylation of H3-K4 at the ADH1 and CUP1 genes. Wild-type and UBP8 deletion mutant strains were grown and cross-linked as described in the legend to Fig. 4. Immunoprecipitations were performed as described in the legend to Fig. 6. Primer pairs targeting the UASs, core promoters, and ORFs of ADH1 and CUP1 were used for PCR analysis of the immunoprecipitated DNA samples. (B) Analysis of H3-K9/14 acetylation at ADH1 and CUP1. Immunoprecipitations were performed as described in the legend to Fig. 6. (C) Analysis of H3-K4 di- and trimethylation at ADH1 and CUP1 in the Δubp8 mutant. Immunoprecipitations were performed as described in the legend to Fig. 6.