FIG. 4.
Mitotic defects in Rb1ΔL/ΔL MEFs. (A) DAPI-stained mitotic figures from wild-type and Rb1ΔL/ΔL cells are shown. The percentage of mitoses with lagging chromosomes was tabulated for at least 100 anaphases in each of three independent experiments. (B) Propidium iodide-stained cells were analyzed by flow cytometry for cells with greater than 4 N DNA content to identify aneuploid cells. The percentages of cells in this category are indicated above the bars. The error bars indicate 1 standard deviation from the mean.