FIG. 7.

PKCδ regulates a posttranslational modification of topoisomerase IIα. (A) MOLT-4 cells were left untreated or treated with aphidicolin or nocodazole for 16 h in the presence (+) or absence (−) of rottlerin. Total RNA was subjected to RT-PCR analysis using primer sets for topoisomerase IIα (TopoIIα) or β-actin. (B) 293T cells were stably transfected with GFP vector (293T/GFP) or GFP-topoisomerase IIα (293T/GFP-TopoIIα). Cell lysates were analyzed by immunoblotting (IB) with anti-GFP or antitubulin. (C) 293T/GFP-TopoIIα cells were treated with aphidicolin or nocodazole for 16 h in the presence or absence of rottlerin. After the cells were washed with phosphate-buffered saline twice, cells were mounted with Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI; Vector Laboratories) and analyzed with a Nikon Eclipse TE2000-U microscope. The cell cycle was determined by using a FACscan. DMSO, dimethyl sulfoxide.