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. 2006 May;26(9):3541–3549. doi: 10.1128/MCB.26.9.3541-3549.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 4. — Cultures of embryos derived from mNle^+/⁻ intercrosses. E3.5 blastocysts were harvested and cultured for 3 days. mNle^+/+ and mNle^+/⁻ blastocysts (A) hatched from the zona pellucida after 1 day and attached to the culture dish. During the following days, the ICM proliferated (arrows) and the trophectoderm differentiated (arrowheads). mNle^−/− blastocysts were indistinguishable from controls at E3.5 (B and C). After 24 h in vitro, most of them were unable to hatch from the zona (C) and degenerated rapidly. The remaining mNle^−/− embryos hatched (B) and attached to the dish, and the trophectoderm expanded and differentiated (arrowhead). However, after 2 days in culture, the ICM degenerated and was no longer discernible. Bars, 50 μm.