TABLE 1.
Time of analysis | No. (%) of live births and dissected embryos from mNle+/− intercrosses from genotype:
|
||
---|---|---|---|
+/+ | +/− | −/− | |
Postnatal | 22 (32) | 46 (68) | 0 |
E10.5-E12.5 | 14 (29) | 34 (71) | 0 |
E6.5 | 9 (29) | 22 (71) | 0 |
E3.5 | 13 (20) | 39 (59) | 14 (21) |
Mice and E6.5 to E12.5 embryos were genotyped by PCR, and E3.5 embryos were genotyped by nested PCR (see Fig. 3C).