FIG. 8.
Clonogenic survival of Rad18+/+ and Rad18−/− cells after genotoxin treatment. Exponentially growing cultures of Rad18+/+ or Rad18−/− MEFs were treated with BPDE (BP; 100 nM or 600 nM), HU (2 mM for 1 h or 2 h), IR (4 Gy or 8 Gy), or UVC (10 J/m2 or 20 J/m2). Twenty-four hours after treatment, cells were trypsinized and counted and then replated at a density of 1,000 cells/10-cm plate. The resulting plates were returned to the incubator and given fresh growth medium every 3 days. After 7 days, cells were fixed and stained with Giemsa. Giemsa-stained colonies containing >50 cells were scored.