Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 May;26(9):3492–3504. doi: 10.1128/MCB.26.9.3492-3504.2006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, American Society for Microbiology

PMC Copyright notice

FIG. 5. — p53-dependent gene repression is not entirely mediated through association with the promoters of repressed genes. (A) MEFs (p53^+/+ and p53^−/−) were exposed to hypoxia or adriamycin for 8 h as described previously. The mRNA levels of Ankyrin-like repeat protein are shown. The 18s rRNA is shown as a loading control. The putative p53 response element identified in the Ankyrin-like repeat protein promoter is also shown. Those base pairs matching the canonical sequence for a p53 response element are underlined. (B) MEFs (p53^+/+ and p53^−/−) were treated as described for panel A. ChIP analysis was carried out using a p53 antibody (Ab), followed by PCR for the Ankyrin-like repeat protein promoter. (C) The binding of p53 to the Ankyrin-like repeat protein gene promoter was compared in primary and transformed MEFs. (D) MEFs (p53^+/+ and p53^−/−) were treated as described for panel A; the mRNA levels of mcm5 are shown. The 18s rRNA is shown as a loading control. (E) MEFs (p53^+/+ and p53^−/−) were treated as described for panel A. ChIP analysis was carried out using a p53 antibody, followed by PCR for the Cdc25C, mcm5, Runx 2, dnmt1, and survivin promoters.