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. 2006 May;26(9):3649–3658. doi: 10.1128/MCB.26.9.3649-3658.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 5. — Recruitment of Hat1p relative to other markers of DNA double-strand break repair. (A) ChIP assays using antibodies that specifically recognize histone H2A serine 129 phosphorylation, histone H4 serine 1 phosphorylation, and Rad52p (RAD52-myc fusion; SQY427) were performed as described in the legend to Fig. 1. Immunoprecipitations for histone H2A serine 129 phosphorylation, histone H4 serine 1 phosphorylation, and Rad52p were replicated three, two, and three times, respectively. (B) PCRs were quantitated and analyzed as described in the legend to Fig. 1.