FIG. 1.

Growth curves of L. lactis MG1363 and its bcaP deletion mutant. Growth rates of L. lactis MG1363 and MGbcaP in CDM supplemented with Casitone (closed and open circles, respectively) are shown. L. lactis MG1363, MGbcaP, and MGbcaP overproducing BcaP were grown in CDM containing free amino acids as the sole source of amino acids (closed triangles, open triangles, and closed squares, respectively). Alternatively, L. lactis MGbcaP was grown in CDM⁷⁵, a medium in which the amino acid concentration was lowered to 75% of that in CDM (open squares). Cells were grown at 30°C, and the absorbance of the culture at 595 nm was measured every 30 min. Shown are the means of at least two independent experiments.