TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype or genotype | Source or reference |
|---|---|---|
| L. lactis strains | ||
| MG1363 | Lac− Prt−; plasmid-free derivative of NCDO712 | 13 |
| NZ9000 | MG1363 pepN::nisRK | 31 |
| NZ9700 | Nisin-producing transconjugant of MG1363 containing Tn5276 | 30 |
| MGbrnQ | MG1363 derivate, chromosomal deletion of brnQ | This work |
| MGbcaPbrnQ | MG1363 derivate, chromosomal deletion of bcaP and brnQ | This work |
| MGbcaP | MG1363 derivate, chromosomal deletion of bcaP | This work |
| Plasmids | ||
| pNZ8048 | Cmr; expression vector carrying the nisin-inducible PnisA | 26 |
| pNZ9530 | Eryr; nisRK cloned in pIL252; constitutive expression of nisRK | 26 |
| pVE6007 | Cmr; temperature-sensitive replication derivate of pWV01 | 40 |
| pORI280 | Eryrori+ RepA−; lacZ expressed constitutively from P32 promoter | 38 |
| pNGbcaP | pNG8048 containing bcaP gene | This work |
| pNGbcaP-H6 | pNG8048 containing bcaP-his6 of L. lactis MG1363 behind PnisA | This work |
| pORIΔbcaP | Eryr LacZ+; pORI280 containing bcaP deletion construct | This work |
| pORIΔbrnQ | Eryr LacZ+; pORI280 containing brnQ deletion construct | This work |
| pORIΔbcaPbrnQ | Eryr LacZ+; pORI280 containing bcaP and brnQ deletion construct | This work |