TABLE 2.
Oligonucleotides used in this study
| Name | Sequence (5′-3′)a |
|---|---|
| ctrA-P1 | GCTCTAGACTAGAATTAGGACATATATCAC |
| ctrA-P2 | CGCGGATCCCTCATAAATCCCATAATAAATCCTC |
| ctrA-P3 | CGCGGATCCTGGTTCCTTATTGGAATTGCG |
| ctrA-P4 | CCGGAATTCAACGGCTGGTACGATACGAAC |
| brnQ-P1 | GCTCTAGACATTAGTCCAAATGGCGATACC |
| brnQ-P2 | CGCGGATCCGATAGTCTTTACCAGCTAGTTTC |
| brnQ-P3 | CGCGGATCCGAGCTTCTAATATTTAGGAGCTC |
| brnQ-P4 | CCGGAATTCCATCTCGGTTTTAACGTCTGAAC |
| ctrA-N | CTAGACCACCATGGGATTTATGAGAAAAGCC |
| ctrA-C | CTAGTCTAGACGTCTTATTTCTTTTTGCGACG |
| ctrA-CH6 | CTAGTCTAGATTAGTGATGGTGATGGTGATGTTTCTTTTTGCGACGATTTCCATAA |
a
Restriction enzyme sites are underlined.