TABLE 2.
Strain (phenotype) | CFU/colonya | % Survivalb |
---|---|---|
ATCC 14028 (wild type+) | ||
Initial colony | 2.3 × 109 ± 0.14 × 109 | |
3 mo desiccation | 1.6 × 109 ± 0.15 × 109 | 68 |
9 mo desiccation | 2.2 × 108 ± 0.19 × 108 | 9.7 |
ΔagfD (fimbriae−, cellulose−) | ||
Initial colony | 3.3 × 109 ± 0.14 × 109 | |
3 mo desiccation | 2.0 × 108 ± 0.28 × 108 | 6.2 |
9 mo desiccation | 8.7 × 106 ± 1.4 × 106 | 0.27 |
ΔagfA (fimbriae−) | ||
Initial colony | 3.2 × 109 ± 0.64 × 108 | |
3 mo desiccation | 3.4 × 108 ± 0.12 × 108 | 11 |
9 mo desiccation | 3.8 × 107 ± 0.34 × 107 | 1.2 |
ΔbcsA (cellulose−) | ||
Initial colony | 2.3 × 109 ± 0.18 × 109 | |
3 mo desiccation | 4.8 × 108 ± 0.37 × 108 | 21 |
9 mo desiccation | 1.6 × 108 ± 0.17 × 107 | 6.8 |
Average number of viable cells (CFU) per dried colony plus or minus the standard error. At least four individual colonies were tested.
Percent survival of cells in desiccated colonies determined relative to values from initial colonies.