TABLE 1.
E. coli strains and plasmidsa
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)205 λ−rpsL150 flbB5301 relA deoC1 pstF25 | CGSC |
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 supE44 hsdR17(rK− mK−) thi-1 gyrA (Nalr) | BRL |
| MG1655 | F−λ−rph-1 | CGSC |
| AB1133 | F−thr-1 ara-14 leuB6 lacY1 (gpt-proA)62 supE44 galK2 λ-rac hisG4 rfbD1 rpsL31 (smr) kdgK51 xyl-5 mtl-1 argE3 thi-1 | CGSC |
| XL1-Blue | recA1 endA1 gyrA96 thi hsdR17(rK− mK−) supE44 relA1 lac [F′proAB+lacIqZΔM15::Tn10 (Tetr)] | Stratagene |
| AB259 | HfrH supQ80 λ−relA1 spoT1 thi-1 | CGSC |
| EZE100 | MC4100 sbmA::Tn5 | This work |
| SBG303 | MC4100 fhuA::Tn5 | 14 |
| RYC1000 | F−araD139 ΔlacU169 Δrbs7 rpsL relA thiA recA56 gyrA | CGSC |
| Plasmids | ||
| pTUC342 | pBR322 mcjA, Apr | |
| pTUC341 | pBR322 mcjBCD, Apr | |
| pEVA100 | pTUC342 mcjA (His5xAla) | This work |
| pEVA101 | pTUC342 mcjA (His5xLys) | This work |
| pEVA102 | pTUC342 mcjA (His5xArg) | This work |
| pEVA103 | pTUC342 mcjA (Gly2xAla) | This work |
| pMM73.4 | pBR322 carrying sbmA+, Tcr | F. Moreno |
a
CGSC, E. coli Genetic Stock Center; BRL, Bethesda Research Laboratory; Apr, ampicillin resistant; Tcr, tetracycline resistant.