TABLE 2.
Effect of MccJ25 mutants on several E. coli strainsa
| Strain | Sensitivity by spot-on-lawn test (microcin MIC, μM)
|
|||
|---|---|---|---|---|
| MccJ25 | MccJ25 (His5xLys) | MccJ25 (His5xArg) | MccJ25 (His5xAla) | |
| MC4100 | 0.9b | 7.3b | Rc | R |
| AB259 | 0.9b | 3.6b | R | R |
| RYC1000 | 0.9b | 3.6b | R | R |
| DH5α | 0.9 | 3.6b | 29.3b | 29.3 |
| XL1-Blue | 0.9 | 7.3b | 29.3b | 29.3 |
| AB1133 | 0.4 | 3.6b | 7.3b | 14.6 |
| MG1655 | 0.4 | 3.6b | 7.3b | 29.3 |
a
Representative results of one of several independent experiments are shown.
b
Turbid spot.
c
R, no inhibition was noted in the presence of 100 μM microcin.