TABLE 3.
Sensitivities of washed E. coli MC4100 cells to MccJ25 analoguesa
| Strain | No. of CFU with MccJ25 mutation
|
||
|---|---|---|---|
| None | His5xAla | His5xArg | |
| MC4100 | 30 | 1,930 | 1,920 |
| MC4100, washed | 0 | 1,910 | 1,950 |
| SBG103 (4100 fhuA::Tn5) | 1,930 | 1,970 | 1,930 |
| SBG103 (4100 fhuA::Tn5), washed | 0 | 1,580 | 1,950 |
| EZE100 (MC4100 sbmA::Tn5) | 1,930 | 1,950 | 1,980 |
| EZE100 (MC4100 sbmA::Tn5), washed | 1,900 | 1,970 | 1,800 |
a
The assay was performed by treating about 2 × 103 cells/ml with 38 μM of MccJ25 or its mutant derivatives. Representative results of one of several independent experiments are shown.