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Proton transport into proteoliposomes containing NDH-1 from Klebsiella pneumoniae and the F1Fo ATPase from Escherichia coli. Proton transport was followed by the quenching of ACMA fluorescence in 10 mM Tris-HCl, pH 7.0, containing 10 mM MgCl2, 50 mM KCl, 10% glycerol, and 5 mM NaCl. The arrows indicate the addition of 0.1 mM NADH or 2.5 mM ATP.
