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. 2006 May;188(9):3264–3272. doi: 10.1128/JB.188.9.3264-3272.2006

FIG. 4.

FIG. 4.

pH optimum for the modification of NDH-1 by DCCD. NDH-1 obtained by PEG precipitation was adjusted to the desired pH with Good buffers and incubated with 698 μM DCCD for 3 min. The control mixtures contained 6 μl ethanol. The residual Na+ concentration was 0.6 mM. The Q reductase activity of NDH-1 modified with DCCD (▾) or without DCCD added (▪) was determined at pH 8.0.