FIG. 6.

DCCD inhibition of Na⁺ transport by NDH-1. NDH-1 obtained by PEG precipitation was reconstituted into proteoliposomes at pH 7.5 and incubated with 200 μM (○), 500 μM (▪), or 800 μM (▾) DCCD or without DCCD (•). The protein-to-lipid ratio was 1:1. Na⁺ transport was initiated by the addition of 5 mM NaCl and 0.1 mM NADH. The data were corrected by subtracting the Na⁺ content of proteoliposomes from a control reaction without NADH added (40 to 80 nmol Na⁺ mg⁻¹ lipid) (Fig. 2).