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Protection of NDH-1 from DCCD inhibition by Na+. NDH-1 obtained by PEG precipitation (0.14 mg in 20 μl 1 M MES, pH 6.5) was mixed with 698 μM DCCD, and the quinone reduction activity was monitored after different reaction times (final DCCD concentration in the assay buffer, 14 μM). The arrow indicates the addition of DCCD at time zero. ○, with DCCD added; •, without DCCD added; ▾, 50 mM NaCl added prior to DCCD addition.
