TABLE 1.

Identification of proteins in NDH-1 from the source 15Q chromatographic step^a

Swiss-Prot accession no.	Protein	Gene	Predicted mass (Da)	No. of peptide matches	Protein score
Q8FH47	Hypothetical protein YdiJ	ydiJ	113,164	2	39
Q8ZLT3	Polynucleotide phosporylase	pnp	76,991	13	589
P0A1H3	Elongation factor G	fusA	77,419	10	425
Q8FAL6	Fumarate reductase flavoprotein subunitb	frdA	65,948	5	130
Q8FAL6	Fumarate reductase flavoprotein subunitb	frdA	65,948	5	106
P0A1Y7	NADH dehydrogenase I, subunit NuoCD	nuoCD	68,817	4	88
O66026	60-kDa chaperonin	groL	57,003	18	602
Q8XG95	ATP synthase alpha subunit	atpA	55,079	15	644
P0ABB4	ATP synthase beta subunit	atpD	50,163	19	671
P0A6N1	Elongation factor Tu	tufA	43,286	6	201
Q7WRJ3	1,3-Propanediol oxidoreductase	dhaT	41,518	2	73
Q8ZH40	d-Methionine binding protein	metQ	29,359	2	83
Q83BR3	NADH dehydrogenase I, subunit NuoI	nuoI	18,985	1	29

^aPolypeptides subjected to tryptic digestion and mass spectroscopic analysis are indicated in the SDS-PAGE gel shown in Fig. 1. Results were compared with the UniRef100 database (http://www.ebi.ac.uk/uniref/) using the Mascot search engine (Matrix Science Ltd., United Kingdom). In cases where the identified K. pneumoniae protein is not deposited in the Swiss-Prot database, the accession number of the corresponding enterobacterial protein with the highest sequence similarity is given as a reference.

^bBoth polypeptides separated by SDS-PAGE (Fig. 1) were assigned to frdA.