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. Author manuscript; available in PMC: 2006 Apr 26.
Published in final edited form as: Exp Cell Res. 2005 Aug 15;308(2):364–380. doi: 10.1016/j.yexcr.2005.04.035

Fig. 3.

Fig. 3

Activation of Rho/Rac GTPases by DmVav proteins. (A–D) COS1 cells were transiently transfected with the combination of proteins indicated at the top of each panel. After transfection, the GTP-bound levels of Rac1 (A, D), RhoA (B) and Cdc42 (C) were evaluated by pull down assays with specific GST bait proteins. (A–C) Left panels, result of the pull-down experiments using anti-AU5 immunoblots. Right panels, levels of expression of the GTPases in the lysates used for the pull-down experiments. (D) Left panel, result of the pull-down experiment using anti-AU5 immunoblots. Right panels, expression of Rac1 (upper panel) and DmVav proteins (lower panel) used in this study. The migration of the GTPases is indicated by arrows. TCL, total cellular lysate.