Fig. 4.

Morphological change induced by Vav family proteins in NIH3T3 cells. Cells were transfected with plasmids encoding the indicated Vav proteins (left side). After transfection, cells were stained with rhodamine-phalloidin and, in the case of DmVav-expressing cells (panels A–I), immunostained with anti-Myc antibodies followed by FITC labeled-secondary antibodies. Cells were then subjected to confocal immunofluorescence analysis. The localization of F-actin (panels A, D, G, J, M, P) and Vav proteins (panels B, E, H, K, N, Q) is shown in red and green, respectively. The areas of co-localization are shown in yellow (panels C, F, I, L, O, R).