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. 2006 Apr 5;34(6):1925–1934. doi: 10.1093/nar/gkl116

Figure 2.

Figure 2

Total tRNA extracted from the the B.subtilis strain BS1045 with a disrupted trmB gene is a substrate for the affinity-purified BsTrmB MTase. Total tRNA (5 µg) was incubated with the purified BsTrmB enzyme (5 µg) in the presence of 30 µM [methyl-14C]AdoMet in TM buffer. After 30 min incubation at 37°C the tRNA was recovered and digested with nuclease P1. The resulting nucleotides were analyzed by 2D-TLC and autoradiography as described in the legend of Figure 1.