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. 2006 Apr 5;34(6):1892–1899. doi: 10.1093/nar/gkl118

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© The Author 2006. Published by Oxford University Press. All rights reserved

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MiR167 control of ARF8-regulation of OsGH3-2. (A) Increased level of cellular miR167 upon delivery of synthesized miR167. To cells growing in auxin-containing medium, 100 nM of artificially synthesized miR167 was delivered and its cellular level was measured at 4 h after the delivery (lane 4). Lane C4 represents the PBS-treated control. (B) RNA blot assay of OsGH3-2 mRNA. Northern hybridization was performed using the same total RNAs used in A using OsGH3-2 coding region as the probe. (C) Increased cleavage of ARF8 mRNA. The same cells, used in (A and B), were examined for the cleavage of ARF8 mRNA by primer extension. The 117 bp 3′-cleavage products of ARF8 mRNA increased in miR167-treated cells (lane 4), compared with the untreated control (lane C4). The upper arrow and the bottom arrowhead represent the full-size mRNA and the 3′ end cleavage product, respectively.