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. 2006 Apr 28;2(4):e34. doi: 10.1371/journal.pcbi.0020034

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© 2006 Kuo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Predicted splice junctions of the 12 selected candidates. First digit of the candidate ID indicates the tag type; the second digit is arbitrarily assigned.

(B) Except for Candidates 1–1, 1–2, and 1–6, all candidates show the correct product size and were sequence validated. A larger band from an unpredicted novel splice junction was also observed for candidate 1–4. Larger bands were also observed for candidates 1–7 and 1–8, but were shown to be known splice variants. Candidates that were validated by RT-PCR and by sequencing are indicated by a check mark (✓) under the respective lane; candidates not validated, by an ×.

NT, negative control with no RNA template; −RT, negative control with no reverse transcriptase.