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. 2006 Apr 28;2(4):e34. doi: 10.1371/journal.ppat.0020034

Figure 6. Cdc48/p97 Removes Polyubiquitinated Proteins from the Replication Vacuole.

Figure 6

(A) HEK 293 cells were silenced for Cdc48/p97 or p47 and incubated with wild-type L. pneumophila at MOI = 5 for 2 hours in CM, washed, and fixed, or further incubated in CM for 16 h. Intact cells were immunostained for polyubiquitinated proteins and L. pneumophila.

(B) Quantification of polyubiquitin positive vacuoles from (A). Untreated (black), si p47 (white), si Cdc48/p97 (gray). Mean of three replicates ± standard error; at least 100 cells counted per replicate.