TABLE 2.
Loci that genetically interact withhntpeb
| Locus (cytology) | Allele | Genetic interaction (expected direction) |
Molecular identity of gene product |
|---|---|---|---|
| Components of the cytoskeleton | |||
| chickadee (chic) (26A9–B1) | 11 | Su (+) | Profilin; actin polymerization/depolymerization |
| 01320 | Su (+) | ||
| 221 | Su (+) | ||
| k13321 | E (−) | ||
| cactusa(35F9–11) | 4 | Su (−) | Transcription factor; cytoplasmic sequestration of Dorsal |
| 1 | No interaction | ||
| Dynamitin (Dmn) (44F6–8) | k16109 | Su (+) | Dynactin motor; microtubule-based movement |
| RhoAa(52E4) | J3.8 | Su (NR) | Rho small monomeric GTPase |
| E3.10 | No interaction | ||
| karst (kst) (63C5–D1) | 01318 | Su (+) | βHeavy-spectrin; actin binding, microtubule binding |
| rolling pebbles (rols) (68F1) | 08232 | E (−) | Component of the cytoplasm; involved in myoblast fusion |
| Extracellular matrix component | |||
| viking (vkg) (25C1) | 01209 | Su (+) | Type IV collagen α2 chain |
| k00236 | Su (+) | ||
| k07138 | Su (+) | ||
| k16721 | Su (+) | ||
| k16502 | Su (+) | ||
| 177-27 | Su (+) | ||
| Cg25C (25C1–2) | k00405 | Su (+) | Type IV collagen α1 chain |
| 234-9 | Su (+) | ||
| Components of signal transduction pathways EGFR signaling pathway | |||
| echinoid (ed) (24D2–4) | k01102 | Su (+) | Contains immunoglobulin domains |
| MESK2 (57E6–9) | k0019 | Su (−) | Suppressor of KSR2; alpha/beta-hydrolase domains |
| EgfRa(57E9–F1) | f1 | E (+) | Epidermal growth factor receptor; protein tyrosine kinase |
| TGFβ/Dpp signaling pathway | |||
| thickveins (tkv) (25C9–D1) | k16713 | Su (+) | Protein kinase; involved in dorsal closure and tracheal system development |
| 09415 | No interaction | ||
| baboon (babo) (44F12–45A1) | k16912 | Su (+) | Type I TGFβ receptor; serine/threonine kinase |
| 32 | No interaction | ||
| JNK signaling pathway | |||
| anterior opena (aop) (22D1) | 1 | Su (NR) | RNA polymerase II transcription factor; transcriptional repressor |
|
Jun related antigen (Jra)a (46E4–5) |
1 | Su (+) | Transcription factor bZIP; Jun related |
| puckered (puc) (84E10–13) | A251.1f3 | Su (−) | Protein tyrosine phosphatase; Jun kinase (JNK) phosphatase |
| Notch signaling pathway | |||
| l(2)44DEa (44D3–6) | k10313 | Su (+) | Acetate-CoA ligase; interacts with l(1)Sc and N |
| 05847 | Su (+) | ||
| Delta (Dl) (92A1–2) | 05151 | E (+) | Notch receptor ligand |
| X | E (+) | ||
| 9P | E (+) | ||
| Other signaling pathways | |||
| plexus (px) (58E3–8) | k08316 | Su (−) | Localized to the nucleoplasm; interacts genetically with Delta, rho, and EGFR |
| k08134 | Su (−) | ||
|
Atypical protein kinase C (aPKC) (51D7–8) |
k06403 | Su (−) | Atypical protein kinase C; mutants affect epithelial apical-basal polarity; associates with Bazooka; expressed apically in tracheae and other epithelia, not in amnioserosa |
| Nucleic acid binding | |||
| vrille (vri) (25D4–5) | k05901 | Su (+) | Transcription factor; bZIP; expressed in amnioserosa, tracheae, eye, and other tissues |
| eIF-4a (26B1–2) | 02439 | Su (+) | RNA helicase; translation initiation factor; expressed ubiquitously in embryos |
| k14518 | Su (+) | ||
| k01501 | No interaction | ||
| dachshund (dac) (36A2) | P | Su (−) | Transcription factor; expressed in CNS and eye disc |
| domino (dom) (57D4–8) | k08108 | Su (−) | Transcription factor; helicase; involved in cell proliferation; expressed in hemocytes and other tissues |
|
defective proventriculus (dve) (58D1–2) |
01738 | Su (−) | Transcription factor; homeodomain |
| k06515 | Su (−) | ||
| apontic (apt) (59F1–2) | k15608 | Su (−) | Transcription factor; expressed in amnioserosa, tracheal system, and other tissues; mutations affect the larval tracheal system and the embryonic heart |
| 09049 | Su (−) | ||
| 03041 | Su (−) | ||
| 06369 | Su (−) | ||
| retained (retn) (59F2–3) | 02535 | Su (−) | DNA-binding protein; expressed in the amnioserosa and the brain |
| slow border cells (slbo) | ry7 | S (+) | Transcription factor; bZIP; required for border cell migration; expressed in border follicle cells, embryonic foregut, midgut, and epidermis |
| ry8 | Su (+) | ||
| 01310 | Su (+) | ||
| reptin (rept) (76A3–4) | 06945 | Su (+) | DNA binding; helicase |
| osa (90C1–2) | 00090 | E (+) | DNA binding; expressed in the eye disc morphogenetic furrow |
| Avr1 | E (+) | ||
| glassa(91A3) | 1 | E (+) | C2H2 zinc-finger transcription factor; eye photoreceptor development |
| 2 | E (+) | ||
| 3 | No interaction | ||
| Localized to cell membranes | |||
| turtle (tutl) (24E1–4) | k14703 | Su (+) | Contains immunoglobulin domains; flight behavior |
| 01081 | No interaction | ||
| lamin (lam) (25E6–F1) | k11511 | Su (+) | Nuclear membrane protein; involved in nuclear envelope reassembly; mutations affect cytoplasmic extensions for terminal cells of the tracheal system |
| 04643 | No interaction | ||
| heixuedian (heix) (35F7–8) | k11403 | Su (−) | Plasma membrane component; integral membrane protein |
| 1 | Su (−) | ||
| Rya-r44F (44F3–8) | k04913 | Su (+) | Ryanodine receptor; caffeine-sensitive calcium release channel; localized to the ER membrane |
| Miscellaneous | |||
| Pdsw (23F3) | k10101 | Su (+) | NADH dehydrogenase |
| Sec61α (26D7–8) | k04917 | Su (+) | Protein transporter |
| Coprox (27C6–8) | k10617 | Su (−) | Coproporphyrinogen oxidase |
| Cyclin E (CycE) (35D4) | 05206 | Su (−) | G1/S specific cyclin |
| k05007 | No interaction | ||
| Sec61β (51B6) | k03307 | Su (−) | Protein transporter; component of the translocon |
| 07214 | Su (−) | ||
|
Proteasome p44.5 subunit (Rpn6) (51C1) |
k00103 | Su (−) | Involved in proteolysis; component of the proteasome regulatory particle |
| bellwether (blw) (59B2) | k00212 | Su (−) | Hydrogen transporting ATP synthase |
| 03972 | Su (−) | ||
| 1 | No interaction | ||
| Thiolase (60A5–7) | k09828 | Su (−) | Acetyl CoA acyltransferase |
| 00628 | No interaction | ||
| non-stop (not) (75D4) | 02069 | Su (NR) | Ubiquitin protease; axonal target recognition |
| eRF1 (77B4–5) | neo28 | Su (+) | Translation release factor involved in termination of protein synthesis |
| Unknown or novel | |||
| l(2)k10001 (25B8–9) | k10001 | Su (+) | Unknown |
| l(2)k00605 (27A1–2) | k00605 | Su (−) | Unknown |
| l(2)k10113 (27F4–6) | k10113 | Su (−) | Unknown |
| l(2)k13905 (36A10–11) | k13905 | Su (−) | Unknown |
| l(2)s1878 (44D5–6) | s1878 | Su (+) | Unknown |
| l(2)00297 (47A13–14) | 00297 | Su (+) | Unknown |
| l(2)k15826 (47C3–4) | k15826 | Su (+) | Unknown; homology to a transtyretin-like protein (BLAST) |
| fs(2)neo12 (48C) | 1 | Su (+) | Unknown |
| unchained (49D1–50D1) | k15501 | Su (+) | Novel; mutations affect the chordotonal organ |
| charlatan (51E2) | 02064 | Su (−) | Novel; transcription factor domains; mutations affect the chordotonal organ and the PNS |
| k04218 | No interaction | ||
| l(2)03605 (57F8–10) | 03605 | Su (−) | Unknown |
| l(2)k13211 (58D6–7) | k13211 | Su (−) | Unknown |
| l(2)k06617 (58D6–7) | k06617 | Su (−) | Unknown |
| l(2)k00611 (58F4–5) | k00611 | Su (−) | Unknown; transcription factor domains |
| l(3)L3930 (75C5–6) | L3930 | Su (+) | Unknown |
| ms(3)neo94 (77B–C) | 1 | Su (+) | Unknown |
| l(3)10615 (85D16) | 10615 | E (+) | Unknown |
| l(3)neo51 (92A) | 1 | Su (−) | Unknown |
All loci are listed that modify the rough eye phenotype exhibited by hntpeb at 29°, including all the P-element lines and other types of mutations. The table is organized by “functional classes.” Su, suppressor; E, enhancer; NR, not relevant (the gene does not map to an interacting region). Plus indicates that the corresponding deficiency showed the same result; minus indicates that it did not show the same result.
a
Additional, candidate gene not within an interacting region defined in Table 1.