TABLE 1.
Longevity assay results
| Mean life span (days)
|
ANOVAb
|
|||||
|---|---|---|---|---|---|---|
| Cross and treatment | Female | Male | Combined | Generations of selectiona | Treatment | Sex |
| Cross 1 | ||||||
| F2 | 26.0 | 28.7 | 27.4 | NA | 0.601 | |
| S | 24.0 | 20.5 | 22.2 | 39 (2.0) | 385.53*** | 4.302 |
| L | 33.8 | 31.5 | 32.7 | 8 (9.4) | ||
| Cross 2 | ||||||
| S | 28.1 | 29.0 | 28.6 | 19 (2.0) | 15.323* | 1.409 |
| L | 33.0 | 34.1 | 33.5 | 5 (7.6) | ||
| Cross 3 (assay 1) | ||||||
| S | 18.0 | 36.0 | 27.0 | 27 (2.1) | 3.056 | 64.265** |
| L | 27.9 | 37.4 | 32.7 | 7 (7.1) | ||
| Cross 3 (assay 2) | ||||||
| S | 18.8 | 31.4 | 25.1 | 48 (2.0) | 41.687** | 7.168 |
| L | 30.1 | 37 | 33.6 | 12 (8.1) | ||
Number of generations of selection preceding the longevity assays. Numbers in parentheses are average generation lengths in weeks.
Numbers are F-values. *P < 0.02, **P < 0.01, ***P < 0.0001. F-values without asterisks were not significant (P > 0.05). For the F2 generation of cross 1, the analysis was by one-way ANOVA of six values (mean male and female life span per replicate cage by three replicate cages) with sex as the factor. All other analyses were three-factor ANOVAs with n = 24 (two treatments × two sexes × three replicate populations/treatment × two cages/population). Treatment (i.e., selection regime) and sex were fixed main effects, and population was a nested random effect within treatment. The F-ratio for the treatment effect was MStreatment/MSpopulation(treatment). The F-test for the sex effect was MSsex/MSsex×population(treatment). Population (treatment) and the interaction effects—sex × treatment and sex × population (treatment)—were not statistically significant (P > 0.05) in all four three-factor analyses.