TABLE 2.
Selection response by cross
| L selection
|
S selection
|
||||||
|---|---|---|---|---|---|---|---|
| Marker | Location | Mean (95% C.I.)a | t-Testb | Mean (95% C.I.)a | t-Testb | Responsec |
Forbes et al. (2004) QTLd |
| A. Cross 1 [S 13 (2.2), L 13 (9.1)]e | |||||||
| AF047180 | 1-0 | 0.59 (0.08) | 4.30* | 0.12 (0.28) | −4.35** | LS | |
| DROPCXGEN | 1-0.9 | 0.55 (0.14) | 0.10 (0.19) | −5.93** | S | ||
| DMSGG3 | 1-1.3 | 0.61 (0.17) | 0.10 (0.19) | −5.93** | S | 1 (3) | |
| DMU56661 | 1-11 | 0.44 (0.09) | 0.09 (0.11) | −10.47*** | S | 1 (3) | |
| AE003438 | 1-18 | 0.43 (0.15) | 0.12 (0.06) | −18.38*** | S | 1 (3) | |
| DROSEV2 | 1-33.4 | 0.57 (0.21) | 0.15 (0.19) | −6.08** | S | ||
| DMTROPONI | 1-57.6 | 0.81 (0.28) | 3.94* | 0.29 (0.10) | −8.80*** | LS | |
| AC004441 | 2-3 | 0.63 (0.24) | 0.22 (0.06) | −18.81*** | S | 2 (1) | |
| AC005555 | 2-31.5 | 0.46 (0.10) | 0.27 (0.17) | −5.35** | S | ||
| AC006302 | 2-48.5 | 0.57 (0.19) | 0.23 (0.02) | −43.28*** | S | 3 (1) | |
| DL | 2-52.9 | — | 0.35 (0.04) | −15.11*** | — | 3 (1) | |
| AC004759 | 2-54.3 | 0.69 (0.15) | 5.30** | 0.34 (0.02) | −27.01*** | LS | 3 (1) |
| AC006472 | 2-61 | 0.71 (0.10) | 7.95*** | 0.35 (0.04) | −15.11*** | LS | 4 (1–3) |
| MAM | 2-70.3 | — | 0.38 (0.17) | −2.98* | — | 4 (1–3) | |
| DMU19731 | 2-71 | 0.72 (0.07) | 12.23*** | 0.38 (0.15) | −3.33* | LS | 4 (1–3) |
| AC004641 | 2-81 | 0.73 (0.13) | 6.92** | 0.46 (0.43) | L | 5 (3) | |
| ELF1 | 2-87 | — | 0.42 (0.30) | — | 5 (3) | ||
| AC004307 | 2-90 | 0.53 (0.19) | 0.37 (0.27) | 5 (3) | |||
| DS08011 | 2-101 | 0.52 (0.10) | 0.45 (0.13) | ||||
| DMRHOb | 3-0.2 | 0.87 (0.11) | 10.56*** | 0.57 (0.38) | L | 6 (2)g | |
| AC004343 | 3-0.5 | 0.86 (0.13) | 9.02*** | 0.56 (0.38) | L | 6 (2) | |
| DMCPDR | 3-1.5 | — | 0.57 (0.38) | — | 6 (2) | ||
| AC004658 | 3-8 | 0.87 (0.22) | 4.86** | 0.54 (0.46) | L | 6 (2) | |
| DMU14395 | 3-18 | 0.82 (0.08) | 15.02*** | 0.43 (0.05) | −5.76** | LS | 6 (2), 7 (1–3) |
| DROLAMB2A | 3-28 | 0.78 (0.08) | 12.89*** | 0.39 (0.17) | L | 7 (1–3) | |
| DMSGS378 | 3-41.4 | 0.59 (0.17) | 0.42 (0.42) | 8 (1, 2), 9 (1–3) | |||
| DRO11DC7Z | 3-47 | 0.71 (0.06) | 13.50*** | 0.43 (0.21) | L | 8 (1, 2), 9 (1–3) | |
| DMTRXIII | 3-54.2 | 0.66 (0.17) | 3.83* | 0.32 (0.18) | −4.18* | LS | 9 (1–3) |
| DRONANOS | 3-67 | 0.45 (0.11) | 0.22 (0.28) | −3.95* | S | ||
| AE003744 | 3-80 | 0.78 (0.29) | 3.50* | 0.16 (0.13) | −9.20*** | LS | 10 (1, 2)h |
| AE003768 | 3-98 | 0.67 (0.19) | 3.79* | 0.18 (0.15) | −7.69*** | LS | |
| B. Cross 2 [S 24 (2.0), L 12 (8.2)]e | |||||||
| DMSGG3 | 1-1.3 | 0.98 (0.06) | 10.55*** | 0.95 (0.11)f | L | 1 (3) | |
| DELTEX | 1-17 | 0.78 (0.20) | 5.42** | 0.32 (0.63) | L | 1 (3) | |
| DMTROPONI | 1-57.6 | 0.18 (0.22)f | 0.16 (0.22) | −5.27** | S | ||
| AE003585 | 2-3 | 0.73 (0.12) | 7.47*** | 0.59 (0.10) | L | 2 (1) | |
| AC005555 | 2-31.5 | 0.53 (0.15) | 0.66 (0.02)f | ||||
| DMU12269 | 2-39 | 0.51 (0.23) | 0.63 (0.11)f | ||||
| CAD | 2-54 | 0.66 (0.21) | 3.20* | 0.44 (0.17) | L | 3 (1) | |
| DROGPAD | 2-60 | 0.68 (0.04) | 18.15*** | 0.43 (0.07) | −3.99* | LS | 4 (1–3) |
| AC006472 | 2-61 | 0.69 (0.03) | 23.00*** | 0.39 (0.07) | −6.98** | LS | 4 (1–3) |
| DMU19731 | 2-71 | 0.71 (0.10) | 7.95*** | 0.24 (0.26) | −3.67* | LS | 4 (1–3) |
| AC004641 | 2-81 | 0.73 (0.12) | 7.47*** | 0.31 (0.21) | −3.77* | LS | 5 (3) |
| DS08011 | 2-101 | 0.61 (0.23) | 0.51 (0.20) | ||||
| AC004343 | 3-0.5 | 0.77 (0.20) | 9.91*** | 0.62 (0.19) | L | 6 (2) | |
| DMU36477 | 3-10 | 0.70 (0.20) | 3.28* | 0.49 (0.12) | L | 6 (2) | |
| DMPROSPER | 3-50 | 0.79 (0.06) | 16.98*** | 0.29 (0.09) | −9.72** | LS | 9 (1–3) |
| DMCP017G | 3-62 | 0.75 (0.14) | 4.39** | 0.51 (0.36) | L | 9 (1–3) | |
| DMTF125 | 3-81.5 | 0.77 (0.10) | 8.24*** | 0.32 (0.03) | −25.09** | LS | 10 (1, 2)h |
| AE003768 | 3-98 | 0.32 (0.02)f | 0.05 (0.08) | −12.01** | S | ||
| C. Cross 3 [S 19 (2.1), L 8 (6.9)]e | |||||||
| DMSGG3 | 1-1.3 | 0.42 (0.10) | 0.35 (0.25) | 1 (3) | |||
| DMU56661 | 1-11 | 0.53 (0.30) | — | — | 1 (3) | ||
| AE003438 | 1-18 | 0.52 (0.14) | 0.36 (0.29) | 1 (3) | |||
| DROSEV2 | 1-33.4 | 0.68 (0.23) | 3.31* | 0.40 (0.29) | L | ||
| DROYP3 | 1-44 | 0.69 (0.09) | 8.45*** | 0.58 (0.15) | L | ||
| DROEXO2 | 1-51.5 | 0.41 (0.08)f | 0.63 (0.06)f | ||||
| AE003588 | 2-1 | 0.66 (0.26) | 0.58 (0.26) | ||||
| DROYANETSB | 2-4 | 0.35 (0.07)f | 0.54 (0.17) | 2 (1) | |||
| AE003615 | 2-22.5 | 0.47 (0.24) | 0.23 (0.23) | 2 (1) | |||
| AC005555 | 2-31.5 | 0.45 (0.21) | 0.41 (0.36) | ||||
| AC006472 | 2-61 | 0.59 (0.16) | 0.36 (0.10) | −6.05** | S | 4 (1–3) | |
| DMMASTER | 2-70.3 | 0.66 (0.10) | 6.66** | 0.29 (0.02) | −34.67** | LS | 4 (1–3) |
| AC004307 | 2-90 | 0.78 (0.02) | 44.28*** | — | — | 5 (3) | |
| AC004365 | 2-97.5 | 0.75 (0.30) | 3.39* | 0.31 (0.10) | −7.63** | LS | |
| AE003482 | 3-13 | 0.61 (0.25) | 0.39 (0.20) | 6 (2), 7 (1–3) | |||
| DMSGS378 | 3-41.4 | 0.52 (0.13) | 0.35 (0.20) | −3.15* | S | 8 (1, 2) | |
| DRO17DC2Z | 3-47 | 0.59 (0.15) | 0.41 (0.06) | −6.36** | S | 8 (1, 2), 9 (1–3) | |
| DROABDB | 3-59 | 0.56 (0.10) | 0.33 (0.14) | −4.87** | S | 9 (1–3) | |
| AE003744 | 3-80 | 0.75 (0.12) | 8.37*** | 0.38 (0.10) | −4.94** | LS | 10 (1, 2)h |
| DROROUGH | 3-91.1 | — | 0.49 (0.02) | — | |||
Frequency of the marker allele from the O (long-lived) parental inbred line used for each cross. Confidence intervals (95%) are given by the mean ± (95% C.I.), where the 95% confidence limit is calculated as t0.05[2] × SEM, and t0.05[2] = 4.303 (two-tailed). Data were not angularly transformed for these calculations.
One-tailed t-test with n = 3 population means, as explained in the text. For L selection, the alternative hypothesis is that the mean O-allele frequency is >0.5. For S selection, the alternative hypothesis is that the mean O-allele frequency is <0.5. Data were angularly transformed for these tests. Results are not shown for tests that were not significant (α = 0.05). *P < 0.05, **P < 0.01, ***P < 0.001.
See text for explanation.
The marker is located within the 95% confidence interval for the indicated QTL of Forbes et al. (2004)(Table 3). The number(s) in parentheses indicates the cross(es) in which the QTL was identified. For example, the marker DMSGG3 is located within the confidence interval for qtl1 of Forbes et al., which they detected in cross 3.
The number of generations of selection that preceded collection of flies for estimation of marker allele frequencies. Numbers in parentheses are mean generation lengths in weeks.
Significantly different from 0.5 in the direction opposite to expectation. This is a two-tailed test using the 95% confidence interval.
Marker located just to the left of the confidence interval for qtl6 (2-0.5–2-25), for which the point location estimate was 2-0.5.
Marker located just to the right of the confidence interval for qtl10, which was between map positions 3-71 and 3-78 (Forbes et al. 2004, Table 3). In all crosses, this is the marker closest to the confidence interval for qtl10.