Figure 4.—

Indirect immunofluorescence location of Sol proteins. Strain SS700 containing pGST-SOL2 or pGST-SOL4, either uninduced (unind) or induced for 1 hr by addition of Cu₂SO₄ to a final concentration of 0.5 mm (ind), YCpSOL1-ET, or pRSSOL1-ET. (Top) Cells were incubated with mouse anti-GST at a dilution of 1:500 or with mouse anti-HA at a dilution of 1:500; Cy3 anti-mouse antibody was used as secondary antibody. (Bottom) The overlay of DNA staining via DAPI and Cy3 detection of the mouse antibodies.