. 2004 Sep;168(1):227–251. doi: 10.1534/genetics.104.026617

TABLE 1.

X and third chromosome regions that modify theDmcycE^JP phenotype

Deficiency	Region removed by deficiency	Effect on DmcycE^JP	Candidate genes in the region
X chromosome
Suppressors
Df(1)tBA1	1A1 to 2A	Suppression	Rbf^a
Df(1)JC19	2F6 to 3C5	Suppression	NC
Df(1)N73 and Df(1)sqh	5D1–2 to 5D5–6	Suppression	roughex, air4 (−)
Df(1)KA14	7F1–2 to 8C6	Mild suppression	lawc (−), air11
Df(1)v^N48	9F to 10C3–5	Suppression	dlg
Df(1)C246	11D–E to 12A1–2	Suppression	BAP60^b
Df(1)RK4	12F5–6 to 13A9–B10	Suppression	NC
Df(1)sc72b	13F1 to 14B1	Suppression	NC
Df(1)ma13	19A1–2 to 20E–F	Suppression	RpS6
Enhancers
Df(1)4b18	14B8 to 14C1	Enhancement	NC
Df(1)N19	17A1 to 18A2	Enhancement	fused
Third chromosome
Suppressors
Df(3L)HR232 and Df(3L)HR119	63C6 to 63D3	Suppression	sprouty
Df(3L)GN24	63F4–7 to 64C13–15	Suppression	cdc4 (ago)^c
Df(3L)vin2 and Df(3L)vin5	68A2–3 to 68D6	Suppression	NC 3.2 region (See Table 5)
Df(3L)brm11	71F1–4 to 72D1–10	Suppression	Brahma3.5 region (see Table 5)
Df(3L)81K19	73A3 to 74F	Suppression	argos, Abl, Dab, sina, sinah^d3.1 region (Table 5)
Df(3R)p712	84D4–6 to 85B6	Suppression	pyd(Z01)
Df(3R)by10	85D8–12 to 85F1	Suppression	hyd (−)
Df(3R)Cha7	90F1–4 to 91F5	Suppression	NC
Df(3R)XS	96A1–7 to 96A21–25	Mild suppression	NC
Df(3R)T1^P	97A to 98A1–2	Mild suppression	l(3)mbt (−), scribble63S15 region (Table 5)
Df(3R)awd^KRB	100C6–7 to 100D3–4	Suppression	tramtrack (−)
Enhancers
Df(3L)emc5	61C3–4 to 62A8	Enhancement	emc (−), trio (S) Rac1^b
Df(3L)R^G7	62B8–9 to 62F2–5	Enhancement	Roughened (Rap1) (−)
Df(3L)HR370	63A1 to 63D10	Enhancement	NC
Df(3L)GN50	63E1–2 to 64B17	Enhancement	cdc2-63E (−), Ras64B^b, RfC40
Df(3L)66C^G28	66B8–9 to 66C10	Enhancement	DNApolα50^b
Df(3L)hⁱ²²	66D10–11 to 66E1–2	Enhancement	h (−), dally (−), mcm7^b
Df(3L)AC1	67A2 to 67D7–13	Enhancement	shc, eif-4E, cdk8^b
Df(3L)Cat	75B8 to 75F1	Enhancement	Replication-deficient region^b
Df(3L)rdgC	77A1 to 77D1	Enhancement	DNA primase
Df(3L)Pc^MK	78A3 to 79E1	Enhancement	cyclin H DNApol-eta^b
Df(3R)Tp110	83C1–2 to 84B1–2	Enhancement	plx
Df(3R)e^N19	93B to 94A	Enhancement	E2F1
Df(3R)crbS87⁴	95E8–F1 to 95F15	Enhancement	crb
Df(3R)3450	98E3 to 99A6–8	Enhancement	string

Where deficiencies that overlap have the same effect on DmcycE^JP, the region common to both deficiencies is given as the cytological region. Candidate genes that are underlined have the same effect on the DmcycE^JP phenotype as the corresponding deficiency. Those indicated by (−) have been tested and shown to have no effect on DmcycE^JP, while those indicated by (S) have been shown to suppress rather than enhance. NC, there was no candidate satisfying our criteria in the interval. Gene descriptions are as follows: Rbf, Retinoblastoma; roughex (inhibitor of Cyclin A/Cdk1 in the MF); air4, aberrant immune response 4 (blood cell tumor suppressor); lawc (enhancer of TxG mutants); air11, aberrant immune response 11 (blood cell tumor suppressor); dlg, discs-large (cytoarchetectual protein, neoplastic tumor suppressor); BAP60, Brahma-associated protein 60 (Brahma complex protein, chromatin remodeling); RpS6, Ribosomal protein S6 (translation factor, tumor suppressor); fused (protein kinase required for Hh signaling); sprouty (acts antagonistically to the Egfr); brahma (SWI2-related ATPase, chromatin remodeling, negative growth regulator); argos (Egfr ligand, anatogonist of Egfr signaling); Abl (nonreceptor tyrosine protein kinase); dab, disabled (acts synergistically with Abl); sina and sinah (ring finger E3 ubiquitin ligases, protein degradation); pyd, ZO1, and tamou (membrane-associated guanylate kinase); hyd, hyperplastic discs (HECT domain E3 ubiquitin ligase, protein degradation); l(3)mbt, lethal (3) malignant brain tumor (translation factor, tumor suppressor); scribble (cytoarchitectual protein, neoplastic tumor suppressor); tramtrack (neural differentiation inhibitor); emc, extra-macrochaetae (Id-related HLH repressor protein required for cell proliferation in the wing and with hairy for MF progression in the eye); trio (Rac-GEF, required for Rac activation); Rac1 (Rac family GTPase); Roughened (Rap1; Ras-like GTPase); cdc2-63E (cdc2-related protein kinase); Ras64B (Ras-related); RfC40, Replication factor-C40 (DNA replication initiation); DNApolα50, DNA polymerase-α 50-kD subunit (DNA replication); h, hairy (see emc); dally (glypican, cooperates with Wg and other growth factor receptors); mcm7, minichromosome maintenance 7 (DNA replication initiation); shc (adaptor protein required for Egfr signaling); eif-4E (translational initiation factor); cdk8 (cdc2-related protein kinase); DNA primase (DNA replication); cyclin H (Cyclin required for activation of cdk8 protein kinase); DNApol-eta (DNA replication); plx, pollux (a cell adhesion protein related to the human oncogene TRE17; Zhang et al. 1996); E2F1 (S-phase transcription factor); crb, crumbs (apical-lateral membrane protein involved in cell polarity); and string (Cdc25 phosphatase, activator of Cdc2). An unidentified gene essential for DNA replication is located within the 75B8–75F1 region (Smith et al. 1993). For more details see text.

^{^a}

Specific alleles of RBF have been shown to suppress the DmcycE^JP phenotype and overexpression of RBF enhances the DmcycE^JP rough eye phenotype (Secombe et al. 1998; our unpublished data).

^{^b}

No specific mutation is available to test the interaction.

^{^c}

cdc4 (ago) alleles have been shown to suppress DmcycE^JP (Moberg et al. 2001).

^{^d}

sina alleles showed only weak dominant suppression of the DmcycE^JP rough eye phenotype; however, a sina-related gene is located next to sina (sinah), and removal of both may account for the suppression observed by the deficiency (M. Coombe, L. Quinn, R. Dickins, J. Secombe and H. Richardson, unpublished results).