TABLE 3.
Interference for nine intervals taken pairwise
| Set | ade5,7-URA3 | URA3-KAN | KAN-lys5 | lys5-NAT | NAT-HYG | HYG-cyh2 | cyh2-crl3 | crl3-trp5 | |
|---|---|---|---|---|---|---|---|---|---|
| A. trp5-leu1 | |||||||||
| I | TT + NPD | 280:217:5 (24.6 ± 1.7) |
319:180:3 (19.7 ± 1.4) |
318:212:1 (20.5 ± 1.2) |
398:137:1 (13.3 ± 1.1) |
455:86:0 (8.0 ± 0.8) |
467:70:3 (8.2 ± 1.2) |
233:303:5 (30.8 ± 1.5) |
473:69:0 (6.4 ± 0.7) |
| PD | 792:607:9 (23.5 ± 0.9) |
810:572:12 (23.1 ± 1.0) |
909:562:4 (19.9 ± 0.7) |
1070:417:5 (15.0 ± 0.7) |
1258:244:0 (8.1 ± 0.5) |
1372:168:3 (6.0 ± 0.5) |
605:861:35 (35.7 ± 1.2) |
727:773:13 (28.1 ± 0.9) |
|
| χ2P | 0.72 | 0.10 | 0.51 | 0.28 | 0.92 | 0.15 | 0.09 | <0.0001 | |
| Sig. SE (cM) | No | No | No | No | No | No | Yes | Yes | |
| II | TT + NPD | 130:323:22 (47.9 ± 2.7) |
292:177:3 (20.7 ± 1.5) |
294:177:2 (19.9 ± 1.4) |
392:87:0 (9.1 ± 0.9) |
379:102:2 (11.8 ± 1.3) |
172:302:8 (36.3 ± 1.9) |
395:85:3 (10.7 ± 1.4) |
|
| PD | 230:641:55 (52.4 ± 2.2) |
562:351:8 (21.7 ± 1.2) |
575:340:9 (21.3 ± 1.2) |
783:143:2 (8.4 ± 0.7) |
764:162:2 (9.4 ± 0.8) |
313:575:40 (43.9 ± 1.9) |
441:482:10 (29.1 ± 1.2) |
||
| χ2P | 0.40 | 0.87 | 0.54 | 0.25 | 0.09 | 0.03 | <0.0001 | ||
| Sig. SE (cM) | No | No | No | No | No | Yes | Yes | ||
| Set | ade5,7-URA3 | URA3-KAN | KAN-lys5 | lys5-NAT | NAT-HPH | HPH-cyh2 | cyh2-crl3 | trp5-leu1 | |
| B. crl3-trp5 | |||||||||
| I | TT + NPD | 446:344:7 (24.2 ± 1.3) |
485:295:6 (21.1 ± 1.2) |
504:326:3 (20.7 ± 1.0) |
614:229:0 (13.6 ± 0.8) |
712:137:0 (8.1 ± 0.6) |
742:104:1 (6.5 ± 0.7) |
465:376:11 (25.9 ± 1.4) |
786:68:1 (4.3 ± 0.6) |
| PD | 622:479:7 (23.5 ± 1.0) |
644:454:9 (22.9 ± 1.1) |
721:447:2 (19.6 ± 0.8) |
848:325:6 (15.3 ± 0.9) |
994:194:0 (8.2 ± 0.5) |
1046:135:5 (7.0 ± 0.7) |
375:789:29 (40.4 ± 1.4) |
727:470:3 (20.3 ± 0.8) |
|
| χ2P | 0.82 | 0.30 | 0.64 | 0.69 | 1.0 | 0.74 | <0.0001 | <0.0001 | |
| Sig. SE (cM) | No | No | No | No | No | No | Yes | Yes | |
| II | TT + NPD | 138:394:39 (55.0 ± 2.9) |
354:212:4 (20.7 ± 1.4) |
353:212:5 (21.2 ± 1.5) |
474:96:1 (8.9 ± 0.9) |
477:94:1 (8.7 ± 0.9) |
266:300:11 (31.7 ± 1.9) |
492:88:0 (7.6 ± 0.7) |
|
| PD | 220:566:38 (48.2 ± 2.1) |
499:312:7 (21.6 ± 1.2) |
511:305:6 (20.7 ± 1.2) |
696:134:1 (8.4 ± 0.7) |
665:167:3 (11.1 ± 0.9) |
223:579:38 (48.0 ± 2.0) |
441:389:6 (25.4 ± 1.2) |
||
| χ2P | 0.15 | 0.88 | 0.95 | 0.76 | 0.09 | <0.0001 | <0.0001 | ||
| Sig. SE (cM) | No | No | No | No | No | Yes | Yes | ||
| Set | ade5,7-URA3 | URA3-KAN | KAN-lys5 | lys5-NAT | NAT-HPH | HPH-cyh2 | crl3-trp5 | trp5-leu1 | |
| C. cyh2-crl3 | |||||||||
| I | TT + NPD | 617:492:5 (23.4 ± 0.9) |
636:465:11 (23.9 ± 1.1) |
742:443:1 (18.9 ± 0.7) |
876:314:4 (14.2 ± 0.8) |
1047:154:0 (6.4 ± 0.5) |
1110:92:4 (4.8 ± 0.6) |
818:382:4 (16.9 ± 0.8) |
895:306:2 (13.2 ± 0.7) |
| PD | 452:328:9 (24.2 ± 1.4) |
495:281:4 (19.6 ± 1.1) |
482:329:4 (21.7 ± 1.1) |
585:239:2 (15.2 ± 0.9) |
659:175:0 (10.5 ± 0.7) |
687:148:2 (9.6 ± 0.8) |
375:456:9 (30.4 ± 1.3) |
605:231:2 (14.5 ± 0.9) |
|
| χ2P | 0.13 | 0.02 | 0.13 | 0.23 | <0.0001 | <0.0001 | <0.0001 | 0.30 | |
| Sig. SE (cM) | No | Yes (neg.) | Yes | No | Yes | Yes | Yes | No | |
| II | TT + NPD | 247:629:45 (48.8 ± 2.0) |
578:328:9 (20.9 ± 1.2) |
577:327:9 (20.9 ± 1.2) |
782:140:2 (8.2 ± 0.7) |
792:137:3 (8.3 ± 0.8) |
617:300:11 (19.7 ± 1.3) |
615:306:4 (17.8 ± 1.0) |
|
| PD | 114:334:32 (54.8 ± 3.1) |
276:201:2 (22.2 ± 1.4) |
291:192:2 (21.0 ± 1.4) |
395:89:0 (9.2 ± 0.9) |
358:128:1 (13.8 ± 1.2) |
223:264:2 (28.2 ± 1.4) |
313:170:2 (18.8 ± 1.4) |
||
| χ2P | 0.22 | 0.051 | 0.22 | 0.17 | <0.0001 | < 0.0001 | 0.50 | ||
| Sig. SE (cM) | No | No | No | No | Yes | Yes | No | ||
| D. HPH-cyh2 | |||||||||
| I | TT + NPD | 125:103:0 (22.6 ± 1.7) |
142:82:3 (22.0 ± 2.7) |
169:72:1 (16.1 ± 1.9) |
195:49:0 (10.0 ± 1.3) |
232:13:0 (2.7 ± 0.7) |
150:91:5 (24.6 ± 3.0) |
140:105:0 (21.4 ± 1.6) |
171:73:0 (15.0 ± 1.5) |
| PD | 944:715:13 (23.7 ± 0.9) |
986:661:12 (22.1 ± 0.8) |
1051:699:4 (20.6 ± 0.7) |
1264:504:6 (15.2 ± 0.7) |
1482:316:0 (8.8 ± 0.5) |
687:1075:35 (35.8 ± 1.1) |
1046:729:13 (22.6 ± 0.8) |
1327:463:4 (13.6 ± 0.6) |
|
| χ2P | 0.34 | 0.38 | 0.01 | 0.01 | <0.0001 | <0.0001 | 0.35 | 0.23 | |
| Sig. SE (cM) | No | No | Yes | Yes | Yes | Yes | No | No | |
| II | TT + NPD | 76:174:16 (50.6 ± 4.1) |
152:113:3 (24.4 ± 2.3) |
191:76:2 (16.4 ± 2.0) |
245:25:1 (5.7 ± 1.4) |
129:135:5 (30.7 ± 2.7) |
170:94:1 (18.9 ± 1.8) |
164:103:1 (20.3 ± 1.8) |
|
| PD | 287:792:60 (50.6 ± 1.9) |
701:421:8 (20.8 ± 1.0) |
681:442:9 (21.9 ± 1.0) |
943:206:1 (9.2 ± 0.6) |
358:747:45 (44.2 ± 1.7) |
665:466:11 (23.3 ± 1.1) |
764:374:5 (17.7 ± 0.9) |
||
| χ2P | 0.43 | 0.24 | 0.004 | 0.001 | <0.0001 | 0.16 | 0.09 | ||
| Sig. SE (cM) | No | No | Yes | Yes | Yes | Yes | No | ||
| Set | ade5,7-URA3 | URA3-KAN | KAN-lys5 | lys5-NAT | HPH-cyh2 | cyh2-crl3 | crl3-trp5 | trp5-leu1 | |
| E. NAT-HPH | |||||||||
| I | TT + NPD | 168:140:4 (26.3 ± 2.3) |
198:111:2 (19.8 ± 1.9) |
224:99:1 (16.2 ± 1.6) |
290:37:0 (5.7 ± 0.9) |
316:11:2 (3.5 ± 1.4) |
175:150:4 (26.4 ± 2.2) |
194:135:2 (22.2 ± 1.8) |
244:85:1 (13.8 ± 1.5) |
| PD | 904:678:9 (23.0 ± 0.8) |
931:636:13 (22.6 ± 0.9) |
999:675:4 (20.8 ± 0.7) |
1179:519:6 (16.3 ± 0.7) |
1482:228:4 (7.4 ± 0.5) |
659:1011:36 (36.0 ± 1.1) |
994:701:11 (22.5 ± 0.8) |
1258:452:3 (13.7 ± 0.6) |
|
| χ2P | 0.26 | 0.29 | 0.002 | <0.0001 | <0.0001 | <0.0001 | 0.99 | 0.92 | |
| Sig. SE (cM) | No | No | Yes | Yes | Yes | Yes | No | No | |
| II | TT + NPD | 65:156:12 (48.9 ± 4.1) |
142:89:2 (21.7 ± 2.3) |
197:36:2 (10.2 ± 2.1) |
207:26:0 (5.6 ± 1.0) |
89:129:13 (44.8 ± 4.4) |
135:92:5 (26.3 ± 3.1) |
145:85:2 (20.9 ± 2.3) |
|
| PD | 296:810:64 (51.0 ± 1.9) |
708:445:9 (21.5 ± 1.0) |
680:485:9 (23.0 ± 1.0) |
943:241:4 (11.2 ± 0.8) |
395:746:36 (40.9 ± 1.5) |
696:467:7 (21.8 ± 1.0) |
783:388:4 (17.5 ± 0.8) |
||
| χ2P | 0.71 | 0.99 | <0.0001 | 0.001 | 0.03 (neg.) | 0.06 | 0.25 | ||
| Sig. SE (cM) | No | No | Yes | Yes | No | No | No | ||
| Set | ade5,7-URA3 | URA3-KAN | KAN-lys5 | NAT-HPH | HPH-cyh2 | cyh2-crl3 | crls3-trp5 | trp5-leu1 | |
| F. lys5-NAT | |||||||||
| I | TT + NPD | 291:219:3 (23.1 ± 1.4) |
321:187:4 (20.6 ± 1.5) |
475:81:2 (8.3 ± 1.1) |
525:37:0 (3.3 ± 0.5) |
510:48:1 (4.8 ± 0.8) |
241:306:12 (33.8 ± 2.0) |
331:225:4 (22.2 ± 1.4) |
422:137:1 (12.8 ± 1.0) |
| PD | 776:591:10 (23.6 ± 0.9) |
795:560:11 (22.9 ± 1.0) |
754:697:3 (24.6 ± 0.7) |
1179:290:0 (9.9 ± 0.5) |
1264:191:4 (7.4 ± 0.6) |
585:850:26 (34.4 ± 1.1) |
848:607:7 (22.2 ± 0.8) |
1070:395:3 (14.1 ± 0.7) |
|
| χ2P | 0.94 | 0.21 | <0.0001 | <0.0001 | 0.006 | 0.36 | 0.72 | 0.28 | |
| Sig. SE (cM) | No | No | Yes | Yes | Yes | No | No | No | |
| II | TT + NPD | 125:372:30 (52.4 ± 2.8) |
406:122:3 (13.2 ± 1.3) |
494:36:2 (4.5 ± 1.0) |
451:77:1 (7.8 ± 1.0) |
194:319:17 (39.7 ± 2.3) |
311:213:4 (22.4 ± 1.5) |
349:177:2 (17.9 ± 1.3) |
|
| PD | 231:590:46 (49.9 ± 2.1 |
452:414:8 (26.4 ± 1.2) |
680:197:0 (11.2 ± 0.7) |
681:188:3 (11.8 ± 0.9) |
291:547:30 (41.9 ± 1.8) |
511:344:9 (23.0 ± 1.3) |
575:290:4 (18.1 ± 1.0) |
||
| χ2P | 0.47 | <0.0001 | <0.0001 | 0.001 | 0.50 | 0.86 | 1.0 | ||
| Sig. SE (cM) | No | Yes | Yes | Yes | No | No | No | ||
| G. KAN-lys5 | |||||||||
| I | TT + NPD | 407:312:4 (23.2 ± 1.2) |
521:203:3 (15.2 ± 1.1) |
700:81:2 (5.9 ± 0.8) |
679:100:0 (6.4 ± 0.6) |
703:72:1 (5.0 ± 0.6) |
333:428:16 (33.7 ± 1.6) |
449:325:4 (22.4 ± 1.1) |
566:213:0 (13.7 ± 0.8) |
| PD | 648:490:8 (23.5 ± 1.0) |
598:548:12 (26.8 ± 1.1) |
809:767:9 (25.9 ± 0.8) |
999:224:0 (9.2 ± 0.6) |
1051:166:3 (7.5 ± 0.6) |
482:720:22 (34.8 ± 1.2) |
721:497:7 (22.0 ± 0.9) |
909:315:3 (13.6 ± 0.7) |
|
| χ2P | 0.92 | <0.0001 | <0.0001 | 0.002 | 0.004 | 0.25 | 0.86 | 0.51 | |
| Sig. SE (cM) | No | Yes | Yes | Yes | Yes | No | No | No | |
| II | TT + NPD | 173:350:23 (44.7 ± 2.5) |
422:121:4 (13.3 ± 1.4) |
454:91:0 (8.4 ± 0.8) |
429:113:3 (12.0 ± 1.3) |
203:319:18 (39.5 ± 2.3) |
319:207:9 (24.4 ± 1.9) |
359:178:2 (17.6 ± 1.3) |
|
| PD | 186:619:53 (54.6 ± 2.3) |
452:399:7 (25.7 + 1.2) |
708:140:2 (8.9 ± 0.8) |
701:151:1 (9.2 ± 0.7) |
276:548:30 (42.6 ± 1.9) |
499:350:4 (21.9 ± 1.1) |
562:288:4 (18.3 ± 1.0) |
||
| χ2P | < 0.0001 | <0.0001 | 1.0 | 0.12 | 0.13 | 0.06 | 0.81 | ||
| Sig. SE (cM) | Yes | Yes | No | No | No | No | No | ||
| Set | ade5,7-URA3 | KAN-lys5 | lys5-NAT | NAT-HPH | HPH-cyh2 | cyh2-crl3 | crl3-trp5 | trp5-leu1 | |
| H. URA3-KAN | |||||||||
| I | TT + NPD | 525:234:4 (16.9 ± 1.1) |
560:204:2 (14.1 ± 1.0) |
571:190:1 (12.9 ± 0.9) |
649:113:0 (7.4 ± 0.6) |
673:83:2 (6.3 ± 0.8) |
285:461:15 (36.2 ± 1.6) |
463:295:6 (21.7 ± 1.3) |
584:182:1 (12.3 ± 0.9) |
| PD | 541:580:9 (28.1 ± 1.0) |
598:519:2 (23.7 ± 0.8) |
795:316:5 (15.5 ± 0.9) |
931:198:0 (8.8 ± 0.6) |
986:139:3 (7.0 ± 0.7) |
495:613:23 (33.2 ± 1.4) |
644:479:6 (22.8 ± 1.0) |
810:317:2 (14.6 ± 0.8) |
|
| χ2P | <0.0001 | <0.0001 | 0.09 | 0.13 | 0.41 | 0.02 | 0.22 | 0.04 | |
| Sig. SE (cM) | Yes | Yes | Yes | No | No | No (neg.) | No | Yes | |
| Set | KAN-lys5 | lys5-NAT | NAT-HPH | HPH-cyh2 | cyh2-cl3 | crl3-trp5 | trp5-leu1 | ||
| I. ade5,7-KAN | |||||||||
| II | TT + NPD | 672:365:8 (19.8 ± 1.1) |
636:394:8 (21.3 ± 1.1) |
874:168:0 (8.1 ± 0.6) |
852:187:3 (9.8 ± 0.8) |
366:639:35 (40.8 ± 1.7) |
604:423:10 (23.3 ± 1.1) |
696:340:5 (17.8 ± 1.0) |
|
| PD | 186:170:3 (26.2 ± 1.9) |
231:122:3 (19.7 ± 1.9) |
296:63:2 (10.4 ± 1.5) |
287:75:1 (11.2 ± 1.3) |
114:233:14 (43.9 ± 3.0) |
220:135:3 (21.4 ± 1.9) |
230:129:1 (18.8 ± 1.5) |
||
| χ2P | 0.0001 | 0.46 | 0.45 | 0.29 | 0.44 | 0.57 | 0.34 | ||
| Sig. SE (cM) | Yes | No | No | No | No | No | No | ||
| Set | URA3-KAN | KAN-lys5 | lys5-NAT | NAT-HPH | HPH-cyh2 | cyh2-cl3 | crl3-trp5 | trp5-leu1 | |
| J. ade5,7-URA3 | |||||||||
| I | TT + NPD | 589:233:4 (15.6 ± 1.0) |
498:315:1 (19.7 ± 0.9) |
601:222:0 (13.5 ± 0.8) |
687:144:0 (8.7 ± 0.7) |
728:100:3 (7.1 ± 0.8) |
336:478:19 (35.5 ± 1.6) |
485:348:3 (21.9 ± 1.0) |
615:220:2 (13.9 ± 0.9) |
| PD | 541:515:10 (27.0 ± 1.1) |
648:404:3 (20.0 ± 0.9) |
776:286:5 (14.8 ± 0.9) |
904:168:0 (7.8 ± 0.6) |
944:122:3 (6.6 ± 0.7) |
452:599:18 (33.1 ± 1.3) |
622:437:9 (23.0 ± 1.1) |
792:278:2 (13.5 ± 0.8) |
|
| χ2P | <0.0001 | 1.0 | 0.92 | 0.37 | 0.69 | 0.49 | 0.41 | 0.89 | |
| Sig. SE (cM) | Yes | No | No | No | No | No | No | No | |