TABLE 1.
AFLP primer combinations used, number of markers generated with each primer combination, and number of polymorphic markers selected for linkage analysis (in parentheses)
|
MseI
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CAA | CAC | CAG | CAT | CTA | CTC | CTG | CTT | Total | ||
| AAC | 50 (9) | 37 (12) | 40 (8) | 37 (6) | 37 (10) | 27 (9) | 40 (6) | 29 (6) | 297 (66) | |
| AAG | 47 (5) | 33 (9) | 23 (4) | 48 (13) | 36 (9) | 25 (7) | 27 (3) | 47 (13) | 286 (63) | |
| ACA | 62 (24) | 27 (7) | 18 (6) | 49 (16) | 47 (17) | 21 (8) | 39 (6) | 21 (10) | 284 (94) | |
| EcoRI | ACC | 17 (3) | 22 (6) | 31 (11) | 13 (3) | 23 (4) | 7 (1) | 15 (3) | 19 (7) | 147 (38) |
| ACG | 25 (5) | 36 (11) | 24 (8) | 15 (4) | 32 (6) | 30 (8) | 16 (4) | 21 (5) | 199 (51) | |
| ACT | 50 (8) | 24 (13) | 22 (5) | 34 (8) | 42 (11) | 15 (3) | 26 (6) | 29 (7) | 242 (61) | |
| AGC | 44 (14) | 22 (3) | 12 (4) | 48 (11) | 17 (4) | 25 (7) | 33 (7) | 38 (8) | 239 (58) | |
| AGG | 20 (6) | 16 (3) | 21 (3) | 33 (5) | 19 (4) | 23 (8) | 25 (8) | 20 (2) | 177 (39) | |
| Total | 315 (74) | 217 (64) | 191 (49) | 277 (66) | 253 (65) | 173 (51) | 221 (43) | 224 (58) | 1871 (470) | |
Only 3′-end selective nucleotides of the primers are shown.