TABLE 1.
Strains and plasmids used in this study
| Genes | Description | Reference |
|---|---|---|
| DR0003 | ddrC (DNA damage response C) | This study |
| DR0070 | ddrB | This study |
| DR0326 | ddrD | This study |
| DR0423 | ddrA | Harris et al. (2004) |
| DRA0346 | pprA | Accession no. O32504 |
| Strains | ||
| Deinococcus radiodurans R1 | ATCC13939 | |
| TNK101 | As R1 but ΔddrC::pkat-aadA | This study |
| TNK102 | As R1 but ΔddrB::pkat-cat | This study |
| TNK103 | As R1 but ΔddrD::pkat-kan | This study |
| TNK104 | As R1 but ΔddrA::pkat-hyg | Harris et al. (2004) |
| TNK105 | As R1 but ΔpprA::pkat-aadA | This study |
| TNK106 | As R1 but ΔrecA::pkat-cat | Harris et al. (2004) |
| TNK107 | ΔddrC::pkat-aadA, ΔrecA::pkat-cat | This study |
| TNK108 | ΔddrB::pkat-aadA, ΔrecA::pkat-cat | This study |
| TNK109 | ΔddrD::pkat-kan, ΔrecA::pkat-cat | This study |
| TNK110 | ΔddrA::pkat-hyg, ΔrecA::pkat-cat | Harris et al. (2004) |
| TNK111 | ΔpprA::pkat-aadA, ΔrecA::pkat-cat | This study |
| TNK112 | ΔddrB::pkat-cat, ΔddrC::pkat-aadA, | This study |
| TNK113 | ΔddrC::pkat-aadA, ΔddrD::pkat-kan | This study |
| TNK114 | ΔddrA::pkat-hyg, ΔddrC::pkat-aadA | This study |
| TNK115 | ΔddrC::pkat-aadA, ΔpprA::pkat-hyg | This study |
| TNK116 | ΔddrB::pkat-cat, ΔddrD::pkat-kan | This study |
| TNK117 | ΔddrA::pkat-hyg, ΔddrB::pkat-cat | This study |
| TNK118 | ΔddrB::pkat-cat, ΔpprA::pkat-hyg | This study |
| TNK119 | ΔddrA::pkat-hyg, ΔddrD::pkat-kan | This study |
| TNK120 | ΔddrD::pkat-kan, ΔpprA::pkat-aadA | This study |
| TNK121 | ΔddrA::pkat-hyg, ΔpprA::pkat-aadA | This study |
| TNK122 | As R1 but ΔddrB::pkat-aadA | This study |
| TNK123 | As R1 but ΔpprA::pkat-hyg | This study |
| DH5α-MCR | F mcrA Δ(mrr-hsdRMS-mcrBC) Φ80 lacZΔ15 ΔlacX74 endA1 recA1 deoR Δ(ara-leu) 7697 araD139 galU galK nupG rpsL |
Invitrogen (Grand Island, NY) |
| Plasmids | ||
| pGEM-T | TA cloning vector | Promega (Madison, WI) |
| pTNK101 | pGEM-T::pkatA-cat | This study |
| pTNK102 | pGEM-T::pkatA-kan | This study |
| pTNK103 | pGEM-T::pkatA-aadA | This study |
| pTNK104 | pGEM-T::pkatA-hyg | This study |
| pTNK201 | pGEM-T::ΔddrC::pkatA-aadA | This study |
| pTNK202 | pGEM-T::ΔddrB::pkatA-cat | This study |
| pTNK203 | pGEM-T::ΔddrB::pkatA-aadA | This study |
| pTNK204 | pGEM-T::ΔddrD::pkatA-kan | This study |
| pTNK205 | pGEM-T::ΔddrA::pkatA-hyg | Harris et al. (2004) |
| pTNK207 | pGEM-T::ΔpprA::pkatA-aadA | This study |
| pTNK208 | pGEM-T::ΔpprA::pkatA-hyg | This study |
| pTNK210 | pGEM-T::ΔrecA::pkatA-cat | Harris et al. (2004) |