Figure 4.—

Genetic analysis of morphogenetic defects in the cln1 and cln2 mutant strains. (A) The cdc42 (DJTD2-16D) and the cdc42cln1 (JCY396) strains were streaked onto YPD agar and incubated at 35° for 3 days. (B) The cdc42 (DJTD2-16D) and the cdc42cln2 (JCY453) strains, transformed with the ptetO:CLN2 plasmid, were streaked onto YPD agar and YPD agar supplemented with 5 μg/ml doxycycline and incubated at 28° for 3 days. (C) Tenfold serial dilutions from exponentially growing cultures of the wild-type (W303-1A), cln1 (JCY275), and cln2 (JCY296) strains were spotted onto YPD agar and YPD agar supplemented with 50 μm latrunculin B and then incubated at 28° for 3 days.