Figure 4.
LMP-420 inhibits replication of M. Tb in human alveolar macrophages (AM). AM were collected by bronchial-alveolar lavage from healthy volunteers and infected overnight in culture with M. Tb (H37Rv; MOI = 1; efficiency of infection = 36 ± 9%). Cells (5 × 105) were plated in 500 μl of medium in a 24-well plate supplemented with nothing, rhuTNF (10 ng/ml), anti-TNF (10 μg/ml) or LMP-420 (10 μM). The first time point for plating to determine CFU was taken immediately after the overnight infection. The second time point was taken after 120 h (108 h after treatment was initiated). Data represent the average ± SEM of thee experiments using 3 different donors.