TABLE 2.
Primers used for the MLVA
| VNTR locus | Forward primer
|
Reverse primer
|
||||
|---|---|---|---|---|---|---|
| Primer name | Sequence | Genome coordinatesa | Primer name | Sequence | Genome coordinatesa | |
| MLVA | ||||||
| VNTR9-1 | NmVNTR09-01FF | ATTAAAGAAACACACGGCG | 391994-392012 | NmVNTR09-01R | GAAAATTCCTGCACAATCC | 392136-392154 |
| VNTR7-2 | NmVNTR07-02FF | AGCAGCATGGTAGCTCGT | 2161864-2161881 | NmVNTR07-02R | GAAGGAAAATCCGGCAA | 2162011-2162027 |
| VNTR7-1 | NmVNTR07-01FF | GGAATGACGGAATCTTAAGTT | 1907337-1907357 | NmVNTR07-01R | ATACCCCGAACTGAAAATG | 1907423-1907441 |
| VNTR21-2 | NmVNTR21-02FF | GCTTCCTGAGTCGCGTC | 1518264-1518280 | NmVNTR21-02R | GCGAAAGACCAAGTCAAAGAT | 1518377-1518397 |
| VNTR13-1 | NmVNTR13-01FF | TTATCGGTATCCCTTCTTTTATG | 369330-369352 | NmVNTR13-01R | TWTAAGTTGACATATTGTGCGAT | 369418-369440 |
| VNTR3-2 | NmVNTR03-02FF | TAGCTGTTGCAACAACACTT | 2061499-2061518 | NmVNTR03-02R | TCTTGATACCGGCGTAAG | 2061631-2061648 |
| VNTR6-1 | NmVNTR06-01FH | GAAGAATAGTTGCCATCGTCT | 863490-863510 | NmVNTR06-01R | TTTTGCGCTGTATTGCC | 863552-863568 |
| VNTR4-5 | NmVNTR04-05FF | CCAAAAGMGGAGAGGTATAAA | 1576196-1576216 | NmVNTR04-05R | TCAAAGGTTTGAGCAATTTGAT | 1576528-1576549 |
| HV-MLVA | ||||||
| VNTR4-4 | NmVNTR04-03FH | ATACACCGTATCCGCCG | 1556715-1556731 | NmVNTR04-03R | CAGGAAGGGGGTTATAATTTTTT | 1556807-1556829 |
| VNTR9-2 | NmVNTR09-02FN | AAAGGCAAAAACCGCAT | 285887-285903 | NmVNTR09-02R | GATACCGCCCTGCTGTT | 286003-286019 |
| VNTR4-2 | NmVNTR04-02FF | TGTGTTGGATTTTCCGTTT | 1400191-1400209 | NmVNTR04-02R | TGTCTTTTTCGGGTTAATTTAGT | 1400367-1400389 |
| VNTR4-3 | NmVNTR04-03FF | GCAAAACAGCGATAAAACAGATA | 321164-321186 | NmVNTR04-03R | ACAAAGGAAAATTTCTCATGACA | 321079-321101 |
a
Nucleotide coordinates in N. meningitidis serogroup B genome sequence NC_003112.