TABLE 3.
Sample typea | % of samples
|
|||||||
---|---|---|---|---|---|---|---|---|
SLME PCR
|
ITS1 PCR
|
kDNA PCR
|
Total
|
|||||
C-Pos | C-Neg | C-Pos | C-Neg | C-Pos | C-Neg | C-Pos | C-Neg | |
Old | 18.2 | 81.8 | 72.7 | 27.3 | 90.9 | 9.1 | 81.8 | 18.2 |
New | 49.4 | 50.6 | 77.8 | 22.2 | 90.1 | 9.9 | 85.2 | 14.8 |
Old, filter papers containing the patient samples were stored desiccated at 4°C for 2 to 3 years prior to DNA extraction and analysis by PCR. New, DNA was extracted and analyzed within 14 days of sample receipt.