FIG. 2.
Sensitivity of genogroup-specific RT-LAMP assay for standard RNAs of genotype GI/2 for genogroup I (A) and GII/12 for genogroup II (B). Lanes: M, 100-bp DNA ladder; 1, 104 copies/tube; 2, 103 copies/tube; 3, 102 copies/tube; 4, 101 copies/tube. Positive reaction shows a ladder-like pattern in 3% agarose gel electrophoresis. The turbidity was measured using an LA-320C (Teramecs), and RT-PCR was carried out with primers G1SKF and G2SKR for genogroup I and G2SKF and G2SKR for genogroup II (13). +, positive; −, negative.