TABLE 2.
Oligonucleotide primers used for M-PCR amplification
| M-PCR no. and primer | Sequence (5′ to 3′) | Amplicon size (bp) | Target | A.T. (°C)a | Reference or source |
|---|---|---|---|---|---|
| M-PCR1 | 58 | ||||
| 146-5F | ATCTGATCAGGACGCTTG | 666 | gmhA (BPSL2795b) (capsule) | This study | |
| 146-5R | CACTGCTTCCCGAAAATG | ||||
| 338-B7F | ACTGGAATCGGGAAAAAC | 482 | 338-B7 (near GI5) | This study | |
| 338-B7R | ACGATATTTTTCCGCTGC | ||||
| 520-E42F2 | ATGCCGGCAGCGTCATAGA | 257 | 520-E42 (GI7) | This study | |
| 520-E42R2 | ACAACGCATGCTTACAGTA | ||||
| 520-E33F | GATCCATGACCACGGCCA | 135 | 520-E33 | This study | |
| 520-E33R | AGGCCGAGAGTCTGATTG | ||||
| M-PCR2 | 58 | ||||
| TRANSF | TTTACCGAAGTCATGAGC | 657 | Transposase | This study | |
| TRANSR | TTTGAAGTGCTGGTCGAC | ||||
| 338-2C5F | AGCAATAAGCGGGCAAAA | 403 | 338-2C5 | This study | |
| 338-2C5R | ATCACAAGCTATCCGCAG | ||||
| 520-E35F | CTACTAGCCACTGATTCC | 290 | 520-E35 | This study | |
| 520-E35R | ATAGATCATTCGTCCGAG | ||||
| 520-2G9F | ACCTCGATTTTGCGTCTG | 145 | 520-2G9 | This study | |
| 520-2G9R | AGAATGGCGTGGAGATTG | ||||
| M-PCR3 | 58 | ||||
| 338-2D10F2 | ATGTCGTGCCTCCGTTCA | 320 | 338-2D10 | This study | |
| 338-2D10R2 | ATGAGTCGGATCGGATCA | ||||
| GI11BF | TGTCGTGGCCCGGGGATTTGTA | 238 | BPSL3260b (GI11) | 12 | |
| GI11BR | TATTCGTTGCTTTCGCGTGTGGTC | ||||
| 520-E36F | GTAATGACGCAAGACGCCG | 132 | 520-E36 | This study | |
| 520-E36R | ACGGCCGAACACAAGAAC | ||||
| M-PCR4 | 50 | ||||
| GI12F | GCAATGGAATCGACGCAACATTG | 788 | BPSL3349b (GI12) | 12 | |
| GI12R | GACGCTGGCGGGTATGGGTAAG | ||||
| 338-B3F | AATCAGACACTCGAGGAC | 605 | 338-B3 | This study | |
| 338-B3R | ATAACCTGCTCGATTTTCC | ||||
| 520-2E10F | CTCCACCGTGACGCTAAG | 392 | 520-2E10 | This study | |
| 520-2E10R | GAGCACTTCACGCGTCTG |
a
A.T., annealing temperature.
b
The GenBank accession number is given.