FIG. 1.

C. pneumoniae RNA degradation at 4°C and at room temperature (RT). The input is given in C. pneumoniae IFU; i.e., 0.1, 1, 10, and 100 IFU was added to the BAL pool prior to the extraction. At different time intervals, lysis buffer was added to the aliquots, and the samples were stored at −80°C prior to extraction.