Flow chart showing the different HPV test methods used with different specimens. Symbols: +, paraffin-embedded tissue; ±, fresh frozen biopsy specimen. Proofer, PreTect HPV-Proofer assay; PCR, type-specific PCR (for HPV types 16, 18, 31, 33, 35, 45, 52, and 58, as indicated); n, number of samples tested.