Time course of Leishmania infections detected in 41 beagle dogs by parasitological, serologic, and n-PCR techniques during 32 months of observations following exposure to the 2002 transmission season, starting from October 2002. Results for two dogs that remained negative until they died in October 2003 are not shown in the graph. White bars, negative by all tests; light gray bars, positive by n-PCR only; hatched bars, positive by serology and n-PCR; dark gray bars, positive by n-PCR and culture/microscopy; black bars, positive by all tests; †, dog's death.