TABLE 1.
Primers used in this study
| Primer | Description | Sequence (5′-3′) | Reference or source |
|---|---|---|---|
| A17F | Forward primer in 16S region, 17 bases from 5′ end | GTTTGATCCTGGCTCAG | 25 |
| 785F | Forward primer in 16S region, 785 bases from 5′ end | GGATTAGATACCCTGGTAGTC | 25 |
| 422R | Reverse primer in 23S region, 422 bases from 5′ end of 23S | GGAGTATTTAGCCTT | 25 |
| L189R | Reverse primer in 23S region, 189 bases from 5′ end of 23S | GGTACTTAGATGTTTCAGTTC | 25 |
| 1512R | Reverse primer in 16S region, 1,512 bases from 5′ end of 16S | TACCTTGTTACGACTT | 25 |
| BergF | Forward primer for Bergeyella spp., 1,000 bases from 5′ end of 16S | GACAGCTGTAGAAATACGG | This study |
| 14BitsR1 | Reverse primer for ITS region of Bergeyella clone AF14B | TCAGCACTCGAAAGTGCTCGG | This study |
| 14BitsR2 | Reverse primer for ITS region of Bergeyella clone AF14B | CTTAGTCTCTATTAATCCCTG | This study |
| M13 Universal (−21) | For DNA sequencing | GTAAAACGACGGCCAGT | MBCa |
| M13-2 Reverse | For DNA sequencing | CAGGAAACAGCTATGAC | MBC |
a
MBC, Molecular Biotechnology Core, Lerner Institute, Cleveland, Ohio.