FIG. 1.

Hybridization, with 18 probes, of multiplex PCR products from a representative sample of 23 C. trachomatis-positive specimens. From top to bottom, probes are as shown in numbered rows. The probe concentrations in each set of three rows (top to bottom) are 0.3125, 0.625, and 1.25 pmol (or 0.625 and 1.25 pmol when there are only two rows per probe). Lanes 1, 7, 8, 11, 15, 17, 19, and 20 are negative; lanes 2 to 6, 9 to 10, 12 to 13, 16, 18, and 21 to 23 are cryptic plasmid positive; lanes 21 and 22 are omp1 (CTS1p) negative and so cannot be subtyped; lanes 2 and 10 are omp1 positive but cannot be subtyped; results for the remaining lanes are as follows: 3, D; 4, F; 5, D; 6, D and E; 9, F; 12, J; 13, I;16, D; 18, E; and 23, D.